PMID: 2495768May 1, 1989Paper

Fatty acid cyclooxygenase activity stimulated by transforming growth factor-beta in mouse osteoblastic cells (MC3T3-E1)

Archives of Biochemistry and Biophysics
K SumitaniM Kumegawa

Abstract

Prostaglandin (PG)E2, a bone-resorption factor, was released essentially as the sole arachidonate metabolite by an osteogenic cell line cloned from mouse calvaria (MC3T3-E1). Transforming growth factor (TGF)-beta (1 ng/ml) or epidermal growth factor (EGF) (10 ng/ml) markedly stimulated the endogenous PGE2 synthesis in the presence of 5% newborn bovine serum. The serum could not be omitted even if both TGF-beta and EGF were added simultaneously. The PGE2 synthesis started after a 1-h lag phase, and reached a maximum at about 3 h after the addition of TGF-beta. The presence of TGF-beta enhanced the cyclooxygenase activity (arachidonic acid----PGH2) assayed with the microsomes or the immunoprecipitate from the solubilized enzyme. The TGF-beta-stimulated PGE2 synthesis was blocked by translation and transcription inhibitors. Furthermore, Western blot analysis using anti-cyclooxygenase antibody demonstrated a higher level of cyclooxygenase in the TGF-beta-treated cells than in the nontreated cells. These experimental results suggested an induction of cyclooxygenase by TGF-beta as previously reported for EGF (K. Yokota et al. (1986) J. Biol. Chem. 261, 15,410-15,415).

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Citations

Oct 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C SchmidE R Froesch
Jun 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J R HarrisonC Pilbeam
Nov 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·U H Lerner
Jan 1, 1993·Calcified Tissue International·K M Reich, J A Frangos
Feb 23, 1990·Biochimica Et Biophysica Acta·L KoehlerM Goppelt-Struebe
May 8, 1991·Biochimica Et Biophysica Acta·D L DeWitt
Jan 1, 1991·Pharmacology & Therapeutics·W L SmithD L DeWitt
Jan 1, 1991·Neurochemistry International·N C SchaadM Schorderet
Oct 1, 1992·Prostaglandins, Leukotrienes, and Essential Fatty Acids·A Ristimäki, L Viinikka
Sep 4, 2004·Biochemical Pharmacology·Ayami Kondo, Akifumi Togari
Jul 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·A RistimäkiL Viinikka
Jan 16, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hidefumi InohKeiji Naruse
Jun 1, 1997·Periodontology 2000·Z SchwartzB D Boyan
Jan 16, 2019·Nature Communications·Hao ChenXu Cao
May 9, 1998·General and Comparative Endocrinology·T M MacDougall, G Van Der Kraak
Jun 1, 1995·Prostaglandins, Leukotrienes, and Essential Fatty Acids·S MatsumotoS Miyazaki

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