PMID: 9546663Apr 18, 1998Paper

Fatty acid elongation in yeast--biochemical characteristics of the enzyme system and isolation of elongation-defective mutants

European Journal of Biochemistry
F DittrichE Schweizer

Abstract

Elongation of long-chain fatty acids was investigated in yeast mutants lacking endogenous de novo fatty acid synthesis. In this background, in vitro fatty acid elongation was dependent strictly on the substrates malonyl-CoA, NADPH and a medium-chain or long-chain acyl-CoA primer of 10 or more carbon atoms. Maximal activity was observed with primers containing 12-14 carbon atoms, while shorter-chain-length acyl-CoA were almost (octanoyl-CoA) or completely (hexanoyl-CoA, acetyl-CoA) inactive. In particular, acetyl-CoA was inactive as a primer and as extender unit. The Michaelis constants for octanoyl-CoA (0.33 mM), decanoyl-CoA (0.83 mM) lauroyl-CoA (0.05 mM), myristoyl-CoA (0.4 mM) and palmitoyl-CoA (0.13 mM) were determined and were comparable for fatty acid synthesis and elongation. In contrast, the affinity of malonyl-CoA was 17-fold lower for elongation (Km = 0.13 mM) than for the fatty acid synthase (FAS) system. With increasing chain length of the primer (> or = 12:0), fatty acid elongation becomes increasingly sensitive to substrate inhibition. Due to the activation of endogenous fatty acids, ATP exhibits a stimulatory effect at suboptimal but not at saturating substrate concentrations. In the yeast cell homogenate, the s...Continue Reading

Citations

Oct 20, 2005·Journal of Bioscience and Bioengineering·M Certik, S Shimizu
Apr 24, 2001·Psychological Bulletin·G F LoewensteinN Welch
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·Eckhart Schweizer, Jörg Hofmann
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Nov 2, 2007·Theoretical Biology & Medical Modelling·Fernando Alvarez-VasquezYusuf A Hannun
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