Fatty acid ethyl esters cause pancreatic calcium toxicity via inositol trisphosphate receptors and loss of ATP synthesis

Gastroenterology
David N CriddleO H Petersen

Abstract

Fatty acid ethyl esters are ethanol metabolites inducing sustained, toxic elevations of the acinar cytosolic free calcium ion concentration ([Ca(2+)](C)) implicated in pancreatitis. We sought to define the mechanisms of this elevation. Isolated mouse pancreatic acinar cells were loaded with fluorescent dyes for confocal microscopy to measure [Ca(2+)](C) (Fluo 4, Fura Red), endoplasmic reticulum calcium ion concentration ([Ca(2+)](ER), Mg Fluo 4), mitochondrial membrane potential (TMRM), ADP:ATP ratio (Mg Green), and NADH autofluorescence in response to palmitoleic acid ethyl ester and palmitoleic acid (10-100 micromol/L). Whole-cell patch clamp was used to measure the calcium-activated chloride current and apply ethanol metabolites and/or ATP intracellularly. Intracellular delivery of ester induced oscillatory increases of [Ca(2+)](C) and calcium-activated currents, inhibited acutely by caffeine (20 mmol/L), but not atropine, indicating involvement of inositol trisphosphate receptor channels. The stronger effect of extracellular ester or acid caused depletion of [Ca(2+)](ER), not prevented by caffeine, but associated with depleted ATP, depleted NADH autofluorescence, and depolarized mitochondria, suggesting calcium-ATPase pump ...Continue Reading

References

Sep 1, 1984·The British Journal of Surgery·A P DicksonC W Imrie
Aug 1, 1983·The Journal of Clinical Investigation·L G Lange, B E Sobel
May 1, 1994·Trends in Pharmacological Sciences·B E EhrlichI Bezprozvanny
Apr 4, 1998·The American Journal of Cardiology·S M Grundy
Sep 3, 1999·The Journal of Biological Chemistry·M JabůrekK D Garlid
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·M RaratyO H Petersen
May 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·H KonoR G Thurman
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·M K ParkO H Petersen
Jan 10, 2002·Nature·Karim S EchtayMartin D Brand
Jan 10, 2002·Gastroenterology·Anna S GukovskayaStephen J Pandol
Feb 14, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Zhao LuFred S Gorelick
Feb 19, 2002·The Journal of Physiology·S VoroninaA Tepikin
Jun 18, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jens WernerCarlos Fernández-Del Castillo
Sep 11, 2002·Biochimica Et Biophysica Acta·Daniele PenzoPaolo Bernardi
Dec 19, 2002·Journal of Clinical Gastroenterology·Dhiraj Yadav, C S Pitchumoni
Jan 30, 2004·Diabetes·Sophie RoussetDaniel Ricquier
Apr 20, 2004·The American Journal of Gastroenterology·Cynthia MortonNatalia Udaltsova
Jul 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·David N CriddleRobert Sutton
Jan 6, 2005·The American Journal of Pathology·Xiaoying DengDavid C Whitcomb
Aug 17, 2005·Cell Calcium·Ilya Bezprozvanny
Oct 1, 2005·Science·Akira FutatsugiKatsuhiko Mikoshiba

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Citations

Jun 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Julia V GerasimenkoOle H Petersen
Mar 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Julia V GerasimenkoOleg V Gerasimenko
Jul 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Julia V GerasimenkoOle H Petersen
Nov 19, 2010·The Journal of Biological Chemistry·Anamika M ReedMichael H Nathanson
Mar 5, 2011·The Journal of Biological Chemistry·Abrahim I OrabiSohail Z Husain
Oct 26, 2013·The Journal of Biological Chemistry·Andrew D JamesJason I E Bruce
Aug 25, 2011·Antioxidants & Redox Signaling·David M BoothDavid N Criddle
Aug 17, 2011·Current Opinion in Gastroenterology·Raghuwansh P Sah, Ashok Saluja
Aug 14, 2012·Current Opinion in Gastroenterology·Raghuwansh P SahAshok K Saluja
Sep 9, 2006·Journal of Gastroenterology and Hepatology·Thilo WelschMarkus W Büchler
Sep 9, 2006·Journal of Gastroenterology and Hepatology·David N CriddleOle H Petersen
Sep 9, 2006·Journal of Gastroenterology and Hepatology·David C Whitcomb
Apr 11, 2008·Journal of Gastroenterology and Hepatology·Irina V OdinokovaAnna S Gukovskaya
Sep 14, 2007·Annual Review of Physiology·Ole H Petersen, Alexei V Tepikin
Sep 13, 2008·American Journal of Physiology. Cell Physiology·Erin M BaggaleyJason I E Bruce
Oct 10, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·David N CriddleJoseph R Reeve
Jan 1, 2010·Digestive Diseases·Stephen J PandolAurelia Lugea
Feb 23, 2013·World Journal of Gastroenterology : WJG·Marta Herreros-VillanuevaLuis Bujanda
May 4, 2011·World Journal of Biological Chemistry·Jason Ie Bruce
Feb 23, 2013·Case Reports in Pediatrics·Elpis MantadakisAthanassios Chatzimichael
Jan 24, 2009·Digestive Diseases and Sciences·Atsushi MasamuneTooru Shimosegawa
Apr 17, 2015·Mediators of Inflammation·Wei HuangDavid N Criddle
May 15, 2013·Free Radical Research·P Bhardwaj, R K Yadav
Aug 21, 2013·Free Radical Research·J A ArmstrongD N Criddle
Jul 6, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Shuang PengOleg V Gerasimenko
Jul 6, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·József Maléth, Péter Hegyi
Feb 17, 2009·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·O H Petersen
Sep 28, 2007·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·David N CriddleRobert Sutton
Aug 8, 2006·Current Opinion in Gastroenterology·Stephen J Pandol
Jun 20, 2012·Gastroenterology·Subhankar DolaiHerbert Y Gaisano
Feb 10, 2018·The Journal of Physiology·Oleksiy GryshchenkoOle H Petersen
Feb 26, 2016·World Journal of Gastrointestinal Pathophysiology·Dahn L ClemensShailender Singh
Feb 4, 2010·Nature Reviews. Gastroenterology & Hepatology·Dhiraj Yadav, David C Whitcomb
Jun 28, 2018·Arquivos Brasileiros De Cirurgia Digestiva : ABCD = Brazilian Archives of Digestive Surgery·Enio Rodrigues VasquesLuiz Augusto Carneiro D'Albuquerque
Aug 30, 2008·Journal of Pediatric Gastroenterology and Nutrition·Gilberto BultronSohail Z Husain
Apr 26, 2008·Pancreas·Robert SuttonStephen J Pandol
Oct 14, 2009·Journal of Gastroenterology and Hepatology·Minoti ApteJeremy Wilson

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