Fatty acid oxidation in neonatal hepatocytes: effects of sepsis and glutamine

Nutrition
Seong Chul KimSimon Eaton

Abstract

Little is known about fat use during sepsis during the neonatal period. Intramitochondrial O(2) consumption is inhibited in isolated hepatocytes from suckling septic rats and this impairment is reversed by glutamine. We investigated the effect of neonatal sepsis on fat oxidation and whether glutamine can directly affect fatty acid oxidation. Suckling Wistar rats (11 d) received an intraperitoneal injection of 300 microg/kg of lipopolysaccharide (Escherichia coli 055:B5); controls received normal saline. At 2 h, hepatocytes were isolated. Hepatocytes were incubated at 37 degrees C with 0.5 mM [1-(14)C]palmitate or 0.5 mM [1-(14)C]palmitate plus 10 mM glutamine. After 1 h, the perchloric acid-soluble (14)C-radioactivity (representing mainly ketone bodies) and (14)CO(2) were measured. Hepatocyte O(2) consumption from 0.5 mM palmitate was measured with and without 2.5 ng/mL myxothiazol to estimate intramitochondrial O(2) consumption. There were no significant differences in fatty acid oxidation between control and endotoxemic hepatocytes measured as acid-soluble radioactivity (which represents mainly ketogenesis, plus Krebs cycle intermediates), as (14)CO(2) production, or as the sum of acid-soluble radioactivity plus (14)CO(2) gen...Continue Reading

References

Apr 19, 1991·Journal of Chromatography·K MertensA Vercruysse
Jun 1, 1986·Biochemical Medicine and Metabolic Biology·J H VeerkampA J Wagenmakers
Mar 15, 1994·European Journal of Biochemistry·S EatonK Bartlett
May 20, 1998·Baillière's Clinical Endocrinology and Metabolism·R R Wolfe
Mar 3, 1999·Journal of Pediatric Surgery·M ZamparelliA Pierro
Jan 26, 2000·Journal of Pediatric Surgery·C RomeoA Pierro

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Citations

Dec 25, 2003·The Proceedings of the Nutrition Society·Simon Eaton
Sep 12, 2016·Analytical and Bioanalytical Chemistry·Zhicheng LiuGuowang Xu

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