Fatty Acid Oxidation in Zebrafish Adipose Tissue Is Promoted by 1α,25(OH)2 D3

Cell Reports
Xuyan PengZhan Yin

Abstract

1α,25(OH)2D3(vitamin D3) is crucial for mineral homeostasis in mammals, but the precise effects of 1α,25(OH)2D3in adipose tissue remain to be clarified in vivo. The initial 25-hydroxylation is catalyzed by liver microsomal cytochrome P450 2R1 (CYP2R1), which is conserved in vertebrates. To probe the physiological function(s) of 1α,25(OH)2D3in teleosts, we generated two independent cyp2r1-deficient zebrafish lines. These mutants exhibit retarded growth and increased obesity, especially in the visceral adipose tissue (VAT). These defects could be rescued with 25(OH)D3treatments. ChIP-PCR analyses demonstrated that pgc1a is the target of the vitamin D receptor in the liver and VAT of zebrafish. Significantly decreased protein levels of Pgc1a, impaired mitochondrial biogenesis, and free fatty acid oxidation are also observed in the cyp2r1 mutant VAT. Our results demonstrate that regulation of 1α,25(OH)2D3during lipid metabolism occurs through the regulation of Pgc1a for mitochondrial biogenesis and oxidative metabolism within zebrafish VAT.

Citations

Mar 26, 2019·The Journal of Endocrinology·Folami Y IderaabdullahCatherine Ann Peterson
Oct 8, 2019·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Roger Bouillon, Dan Bikle
Jul 18, 2020·Applied Microbiology and Biotechnology·Shouxiang SunJian Gao
Mar 21, 2019·Animal Models and Experimental Medicine·Francesca FaillaciErica Villa
Sep 5, 2018·Frontiers in Cell and Developmental Biology·Liqing ZangWenbiao Chen
Feb 1, 2019·Biochemical Society Transactions·Talhah M SalmiAndrew G Cox
Dec 7, 2020·Annales d'endocrinologie·Antoine-Guy LopezRachel Desailloud
Dec 7, 2019·The Journal of Steroid Biochemistry and Molecular Biology·Roger Bouillon, Leen Antonio
Mar 7, 2021·The Journal of Steroid Biochemistry and Molecular Biology·Shubin LiuLiqiao Chen

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