PMID: 6103540Apr 1, 1980Paper

Fatty acid-requiring mutant of Saccharomyces cerevisiae defective in acetyl-CoA carboxylase

Proceedings of the National Academy of Sciences of the United States of America
R RoggenkampE Schweizer

Abstract

The isolation and biochemical properties of a Saccharomyces cerevisiae mutant (acc1-167) defective in acetyl-CoA carboxylase [acetyl-CoA:carbon-dioxide ligase (ADP forming), EC 6.4.1.2] activity are described. The mutant is deficient in de novo biosynthesis of long-chain fatty acids and specifically requires a saturated fatty acid of chain length 14-16 C atoms for growth. Fatty acid synthetase levels were normal, but the acetyl-CoA carboxylase specific activity of the purified enzyme was reduced to approximately 5% compared to wild-type yeast. Upon sodium dodecyl sulfate/polyacrylamide gel electrophoresis the purified mutant enzyme migrated as a single band and was essentially indistinguishable from the wild-type enzyme. The study of acetyl-CoA carboxylase partial activities revealed that the biotin incorporation capacity and the transcarboxylase partial activity were unaffected whereas the biotin carboxylase component enzyme exhibited less than 10% of wild-type specific activity. This biotin carboxylase mutational deficiency could be ascribed to a more than 90% reduction of Vmax and to a comparable increase in the Km value for ATP, which was accompanied by an increased requirement for Mg2+. It is concluded that acc1-167 contai...Continue Reading

References

Jan 1, 1977·Annual Review of Biochemistry·H G Wood, R E Barden
Jan 1, 1976·Annual Review of Biochemistry·K Kirschner, H Bisswanger
Oct 1, 1975·European Journal of Biochemistry·G Dietlein, E Schweizer
Jan 1, 1973·Annual Review of Biochemistry·J J Volpe, P R Vagelos
Jan 21, 1972·European Journal of Biochemistry·T W OrmeE Schweizer
Jan 1, 1974·Annual Review of Biochemistry·A J Fulco
Oct 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·E Schweizer, H Bolling
Dec 1, 1959·Journal of Cellular and Comparative Physiology·F LYNEN

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Citations

Nov 15, 1995·Molecular & General Genetics : MGG·A BaileyJ Hargreaves
Oct 1, 1991·Journal of Industrial Microbiology·P K BajpaiO P Ward
Dec 4, 2003·Journal of Theoretical Biology·Fernando Alvarez-VasquezEberhard O Voit
Jun 15, 1990·Biochemical and Biophysical Research Communications·L A Witters, T D Watts
Sep 5, 2006·Biochimica Et Biophysica Acta·Oksana TehlivetsSepp D Kohlwein
Sep 30, 2017·FEMS Yeast Research·Ruben Fernandez-Moya, Nancy A Da Silva
Oct 1, 1980·European Journal of Biochemistry·M MishinaE Schweizer
Aug 2, 1996·The Journal of Biological Chemistry·D A Toke, C E Martin
Nov 11, 2020·Biological Chemistry·Pia Erdbrügger, Florian Fröhlich
Feb 6, 2021·Biological Chemistry·Pia Erdbrügger, Florian Fröhlich
Apr 20, 2021·Metabolic Engineering Communications·Shota IsogaiAkihiko Kondo

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