Fatty acid specificity of T1 lipase and its potential in acylglycerol synthesis

Journal of the Science of Food and Agriculture
Xiao-Li QinBo Yang

Abstract

T1 lipase has received considerable attention due to its thermostability. Fatty acid specificity of T1 lipase (crude and purified) was investigated, and its potential in the synthesis of acylglycerols was also evaluated. Fatty acid specificity of T1 lipase (crude and purified) was investigated in the esterification of fatty acids (C6:0 to C18:3), suggesting that crude and purified T1 lipase had the lowest preference for C18:0 [specificity constant (1/α) = 0.08] followed by C18:1 (1/α = 0.12) and showed the highest preference for C8:0 (1/α = 1). A structural model was constructed to briefly explore interactions between the lipase and its substrate. Furthermore, crude T1 lipase-catalysed synthesis of diacylglycerols (DAGs) and monoacylglycerols (MAGs) by esterification of glycerol with C18:1 was studied for evaluating its potential in acylglycerols synthesis. The optimal conditions were glycerol/oleic acid molar ratio 5:1, the lipase concentration 9.7 U g(-1) of substrates, water content 50 g kg(-1) of substrates and temperature 50 °C, which yielded 42.25% DAGs, 26.34% MAGs and 9.18% triacylglycerols at 2 h. DAGs and MAGs were synthesised in good yields although C18:1 (a much poorer substrate) was used. Our work demonstrates that...Continue Reading

References

Oct 7, 1998·Protein Science : a Publication of the Protein Society·J LiangC Woodward
Apr 12, 2007·Extremophiles : Life Under Extreme Conditions·Thean Chor LeowAbu Bakar Salleh
Oct 13, 2007·Proteins·Hiroyoshi MatsumuraRaja Noor Zaliha Raja Abd Rahman
Dec 6, 2008·The Journal of Biological Chemistry·César Carrasco-LópezJuan A Hermoso
May 7, 2010·Journal of Chemical Information and Modeling·Ying WangJing-Fang Wang
Apr 23, 2011·Journal of Agricultural and Food Chemistry·Xiao Li QinBo Yang
Jan 24, 2012·Journal of Bioscience and Bioengineering·Dongming LanYonghua Wang
Feb 9, 2012·International Journal of Molecular Sciences·Rudzanna RuslanAbu Bakar Salleh
Jun 14, 2012·Protein Science : a Publication of the Protein Society·Mohd Zulhilmi Abdul RahmanThean Chor Leow

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Citations

Apr 4, 2015·International Journal of Molecular Sciences·Dongming LanYonghua Wang
Jul 18, 2016·Journal of Photochemistry and Photobiology. B, Biology·Vijay KumarSyed Hadi Hasan
Aug 30, 2016·Journal of Agricultural and Food Chemistry·Daoming LiYonghua Wang

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