PMID: 4291254Sep 1, 1966Paper

Fatty acid synthesis in cell-free system from rabbit aorta.

Journal of Lipid Research
A F Whereat

Abstract

The objectives of this study were to identify the subcellular fraction responsible for fatty acid synthesis in rabbit aorta and to determine the effect of cholesterol feeding on the system. A method for homogenization of aorta is described which permitted the isolation of subcellular components of aorta, including mitochondria that were morphologically and functionally intact. Mitochondria were identified as the major site of fatty acid synthesis in this tissue. Cofactor requirements and products showed that the synthetic system operates by chain elongation. Mitochondria from atherosclerotic aortas incorporated acetate into fatty acids faster than did mitochondria from control aortas. It is concluded that cholesterol feeding leads to alterations of aortic mitochondrial function and accelerates the fatty acid elongation pathway.

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.