Fatty acid synthesis in Xylella fastidiosa: correlations between genome studies, 13C NMR data, and molecular models

Biochemical and Biophysical Research Communications
Denise OsiroLuiz Alberto Colnago

Abstract

Xylella fastidiosa was the first plant pathogen to have its complete genome sequence elucidated. Routine database analyses suggested that two enzymes essential for fatty acid synthesis were missing, one of these is the holo-acyl-carrier-protein synthase. However, here we demonstrate, using (13)C NMR spectroscopy, that X. fastidiosa is indeed able to synthesize fatty acids from acetate via an apparently conventional metabolic pathway. We further identify a gene product HetI, an alternative phosphopantetheinyl transferase, which we propose to fill the missing link. Homology modeling of HetI shows conservation of the Coenzyme A binding site suggesting it to be an active enzyme and reveals several interesting structural features when compared with the surfactin synthase-activating enzyme, on which the model was built. These include a simplified topology due to N- and C-terminal deletions and the observation of a novel serine ladder.

References

Mar 5, 1992·Nature·R LüthyD Eisenberg
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Feb 10, 1987·Biochemistry·L A ColnagoS J Opella
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Oct 10, 1998·Biochimica Et Biophysica Acta·D A Los, N Murata
Nov 18, 1998·Current Opinion in Structural Biology·R A LaskowskiJ M Thornton
Dec 11, 1999·Nucleic Acids Research·H M BermanP E Bourne
Jan 8, 2000·The Journal of Biological Chemistry·R S FlugelC T Walsh
Sep 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Anamitra BhattacharyyaNikos C Kyrpides
Feb 21, 2003·FEMS Microbiology Letters·Eliana Gertrudes de Macedo LemosJoão Carlos Campanharo
Jan 1, 1996·Annual Review of Phytopathology·A H Purcell, D L Hopkins
Jun 2, 2004·Biochemistry·L A ForatoL A Colnago
Jul 15, 2004·FEMS Microbiology Letters·Denise OsiroMarcos Antônio Machado

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Citations

Apr 25, 2006·Journal of Theoretical Biology·M G Van der LindenS T Farias
Aug 30, 2005·Current Opinion in Microbiology·João C SetubalAna C R da Silva

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