Fatty Acids Have Different Adipogenic Differentiation Potentials in Stromal Vascular Cells Isolated from Abdominal Fat in Laying Hens

Lipids
Alemu RegassaW Y Kim

Abstract

This study was conducted to examine the effects of fatty acids (FA) with/without chicken serum (CS) on the expression of adipogenic transcripts and adipogenesis in chicken stromal vascular cells (SVC). In experiment 1, SVC were grown in DMEM containing 10% FBS (Control) and treated with 300 µM oleic acid (OLA) + FBS, linoleic acid (LNA) + FBS, palmitic acid (PAM) + FBS, or stearic acid (STA) + FBS for 48 h. In experiment 2, cells were grown in DMEM containing 5% CS and treated with 300 µM OLA (CS + OLA), PAM (CS + PAM), STA (CS + STA) or 200 µM LNA (CS + LNA) for 48 h. Adipogenesis was determined using Oil Red O staining and glycerol-3-phosphate dehydrogenase (GPDH) activity. The proportion of OLA, PAM, or STA was increased (P < 0.05) in SVC grown in either FBS or CS with OLA, PAM or STA. Adipogenesis was induced in FBS + OLA, FBS + LNA, FBS + PAM, FBS + STA, CS + OLA, CS + LNA, CS + PAM, or CS + SAT compared to FBS. GPDH activity was significantly higher in FBS + OLA and FBS + LNA than one in FBS. Compared to FBS, the expression of FABP4 mRNA increased (P < 0.05) in FBS + OLA, FBS + LNA, or FBS + PAM, whereas that of C/EBPα, C/EBPβ, and ATGL increased (P < 0.05) in FBS + OLA or FBS + LNA cells. Expression of FABP4 and C/EBPβ m...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P A GrimaldiN A Abumrad
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·B M FormanR M Evans
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·S A KliewerJ M Lehmann
Sep 18, 2002·Journal of Lipid Research·Lionel ClémentPhilippe Besnard
Feb 11, 2003·Journal of Lipid Research·Florence MassieraGérard Ailhaud
Apr 3, 2003·Biochemical and Biophysical Research Communications·Kihwa KangMichael W Pariza
May 6, 2003·Journal of Lipid Research·J Mark BrownMichael K McIntosh
Nov 20, 2004·Science·Robert ZimmermannRudolf Zechner
Jun 1, 2005·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Yusuke MatsubaraYukio Akiba
Jun 14, 2005·Biochimica Et Biophysica Acta·Lise MadsenKarsten Kristiansen
Aug 16, 2005·Journal of Animal Science·T D Brandebourg, C Y Hu
Oct 3, 2006·Cell Metabolism·Stephen R Farmer
Sep 16, 2008·The Journal of Nutritional Biochemistry·Jeong-Yeh YangClifton A Baile
Mar 10, 2009·Trends in Endocrinology and Metabolism : TEM·Martina I Lefterova, Mitchell A Lazar
Apr 21, 2011·Human Genomics·Rebecca L Smathers, Dennis R Petersen
Sep 23, 2011·In Vitro Cellular & Developmental Biology. Animal·Tran Thi Thanh Khuong, Dong Kee Jeong
Mar 29, 2012·Critical Reviews in Food Science and Nutrition·Allison Dilzer, Yeonhwa Park

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