Fatty acids modulate the expression levels of key proteins for cholesterol absorption in Caco-2 monolayer

Lipids in Health and Disease
Fang YangJing Li

Abstract

Fatty acids have been shown to modulate intestinal cholesterol absorption in cells and animals, a process that is mediated by several transporter proteins. Of these proteins, Niemann-Pick C1-Like 1 (NPC1L1) is a major contributor to this process. The current study investigates the unknown mechanism by which fatty acids modulate cholesterol absorption. We evaluated the effects of six fatty acids palmitic acid (PAM), oleic acid (OLA), linoleic acid (LNA), arachidonic acid (ARA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on cholesterol uptake and transport in human enterocytes Caco-2 cells, and on the mRNA expression levels of NPC1L1, others proteins (ABCG5, ABCG8, ABCA1, ACAT2, MTP, Caveolin 1, Annexin-2) involved in cholesterol absorption, and SREBP-1 and SREBP-2 that are responsible for lipid metabolism. The polyunsaturated fatty acids (PUFAs), especially for EPA and DHA, dose-dependently inhibited cholesterol uptake and transport in Caco-2 monolayer, while saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) had no inhibitory effects. EPA and DHA inhibited cholesterol absorption in Caco-2 monolayer might be caused by down-regulating NPC1L1 mRNA and protein levels, which were associated with inh...Continue Reading

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Citations

Jan 11, 2019·Chinese Medical Journal·Guo-Ming PangCheng Lu
Sep 5, 2019·Molecular Nutrition & Food Research·Barbara WaltherGuy Vergères
Apr 21, 2020·Nutrition Research Reviews·Elif Ulug, Reyhan Nergiz-Unal
Jun 8, 2021·Life Sciences·Paulo Henrique Evangelista-SilvaElizabethe Adriana Esteves

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Methods Mentioned

BETA
PCR
transmission electron microscopy
Assay
Protein Assay
MDA
fatty

Software Mentioned

Quantity One
iQ5
SPSS

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