Fatty acids promote bovine skeletal muscle satellite cell differentiation by regulating ELOVL3 expression

Cell and Tissue Research
Jia-Hui XuYun Qin Yan

Abstract

Fatty acids (FAs) play essential roles in regulating differentiation and proliferation by affecting gene expression in various cell types. However, their potential functions in bovine cells remain unclear. Herein, we examine the differentiation and proliferation of bovine skeletal muscle-derived satellite cells (MDSCs) after incubation with three types of representative FAs (palmitic acid, oleic acid and docosahexaenoic acid) by western blotting, immunofluorescence assays, flow cytometry analysis and EdU incorporation assays. The myotube fusion rate, myotube length and expression levels of muscle differentiation-related gene myogenin (MYOG) and myosin heavy chain 3 (MYH3) increased significantly, although the FAs did not affect proliferation. Additionally, FA-induced bovine MDSC differentiation increased ELOVL3 expression and relocation of ELOVL3 to cytoplasmic lipid droplets in the differentiation of bovine MDSCs. Moreover, the effect of FAs on bovine MDSC differentiation was inhibited upon ELOVL3 downregulation. Collectively, these data indicate that FAs promote bovine MDSC differentiation by regulating ELOVL3 expression.

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Citations

Mar 17, 2020·Journal of Animal Science·Madison L GonzalezSally E Johnson
Sep 30, 2020·Journal of Cellular Physiology·Liyi Wang, Tizhong Shan
Aug 28, 2021·Journal of Animal Science and Technology·Junfang ZhangSeong-Ho Choi

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Methods Mentioned

BETA
flow cytometry
electrophoresis
transfection

Software Mentioned

CXP
GraphPad
ImageJ
Prism

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