PMID: 9187301May 17, 1997Paper

Fatty alcohol synthesis accompanied with chain elongation in liver peroxisomes

Biochimica Et Biophysica Acta
H Hayashi, A Sato

Abstract

We have reported that the fatty alcohol destined to form the 1-alkenyl group of plasmalogen is synthesized as a nascent fatty alcohol within peroxisomes, from acetyl-CoA supplied by beta-oxidation. The present experiment was designed to confirm this peroxisomal fatty alcohol synthesis by using [1-14C]acetyl-CoA. Rats were fed for 2 weeks on chow containing 0.25% clofibrate, as a peroxisome proliferator. Peroxisomes were prepared from the rat liver to more than 85% purity, and incubated with [1-14C]acetyl-CoA in order to investigate suitable conditions for fatty alcohol biosynthesis. Dodecanoyl-CoA was the most suitable primer, and the reaction required NADH and/or NADPH as a reducing factor; NAD+ or NADP+ greatly inhibited the reaction, even if a significant amount of the reduced form still remained. The reaction proceeded linearly with respect to peroxisomal protein concentration and incubation time. Under the optimum conditions for fatty alcohol synthesis, 1 mg of peroxisomal protein could produce approx. 0.7 nmol of fatty alcohol per hour, and the main product was hexadecanol.

References

Sep 1, 1978·Journal of Biochemistry·H Hayashi, T Suga
Jan 1, 1976·Analytical Biochemistry·A Bensadoun, D Weinstein
Oct 1, 1992·American Journal of Respiratory Cell and Molecular Biology·S K Krisans
Nov 1, 1989·Archives of Biochemistry and Biophysics·H Hayashi, A Miwa
Nov 6, 1989·Biochimica Et Biophysica Acta·D Hardeman, H van den Bosch
Mar 1, 1989·The Journal of Clinical Investigation·P A WatkinsM E Beard
Dec 5, 1986·Biochimica Et Biophysica Acta·E ChristensenB O Christophersen
Dec 1, 1987·Archives of Biochemistry and Biophysics·H SinghA Poulos
May 14, 1987·Biochemical and Biophysical Research Communications·U DiczfalusyJ I Pedersen
Sep 4, 1987·Biochimica Et Biophysica Acta·O H Skjeldal, O Stokke
Nov 15, 1986·Analytical Biochemistry·M K Ghosh, A K Hajra
Mar 1, 1986·Archives of Biochemistry and Biophysics·M K Ghosh, A K Hajra
Jan 1, 1982·Annals of the New York Academy of Sciences·A K Hajra, J E Bishop
Jul 1, 1981·Biochemical Pharmacology·H HayashiT Suga
Jan 6, 1995·The Journal of Biological Chemistry·H Sakuraba, T Noguchi
Jan 1, 1993·Biochimie·H van den BoschR B Schutgens

❮ Previous
Next ❯

Citations

May 29, 2004·Biochimica Et Biophysica Acta·Pedro BritesRonald J A Wanders
Oct 31, 1998·Biochimica Et Biophysica Acta·T C Lee
Jun 19, 2010·Journal of Lipid Research·Paul P Van Veldhoven
Jul 5, 2011·Chemistry and Physics of Lipids·Stefan Wallner, Gerd Schmitz
Jul 18, 2009·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Elodie PasturalDayan B Goodenowe
Jun 11, 2008·Journal of Neurochemistry·Mushfiquddin KhanInderjit Singh
Jul 16, 2010·Brain Pathology·Inderjit Singh, Aurora Pujol
Mar 17, 2015·Behavioural Brain Research·Laurent GrégoireThérèse Di Paolo
May 26, 2012·Biochimica Et Biophysica Acta·Nancy E Braverman, Ann B Moser
May 16, 2000·The Journal of Biological Chemistry·A K DasA K Hajra
Sep 29, 2020·Frontiers in Cell and Developmental Biology·Yulong ZhouBing Tian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.