Fatty alcohols production by oleaginous yeast

Journal of Industrial Microbiology & Biotechnology
Sandy FilletJosé L Adrio

Abstract

We have engineered Rhodosporidium toruloides to produce fatty alcohols by expressing a fatty acyl-CoA reductase from Marinobacter aquaeolei VT8. Production of fatty alcohols in flasks was achieved in different fermentation media at titers ranging from 0.2 to 2 g/L. In many of the conditions tested, more than 80 % of fatty alcohols were secreted into the cultivation broth. Through fed-batch fermentation in 7 L bioreactors, over 8 g/L of C(16)-C(18) fatty alcohols were produced using sucrose as the substrate. This is the highest titer ever reported on microbial production of fatty alcohols to date.

References

Dec 14, 2001·The Journal of Biological Chemistry·Line SandagerSten Stymne
Nov 21, 2002·Biochemical Society Transactions·C Ratledge
Aug 19, 2006·Biochimica Et Biophysica Acta·Tibor CzabanyGünther Daum
Feb 9, 2008·Science·Joseph FargionePeter Hawthorne
Dec 9, 2008·Journal of Plant Physiology·Thuy T P DoanPeter Olsson
Mar 6, 2010·Journal of Industrial Microbiology & Biotechnology·Xin ZhaoZongbao K Zhao
Jul 31, 2010·Science·Andreas SchirmerStephen B del Cardayre
Apr 6, 2011·Applied Microbiology and Biotechnology·Jose Manuel AgeitosTomas G Villa
Apr 8, 2011·Angewandte Chemie·Ursula BiermannHans J Schäfer
Nov 22, 2011·European Journal of Lipid Science and Technology : EJLST·Anders S CarlssonPer Hofvander
Oct 11, 2012·Nature Communications·Zhiwei ZhuZongbao K Zhao
Jun 25, 2013·Applied Microbiology and Biotechnology·Aiqiu LiuXuefeng Lu
Oct 22, 2013·Metabolic Engineering·J Tyler YoungquistBrian F Pfleger
Aug 15, 2014·Biotechnology and Bioengineering·Xiaoling Tang, Wei Ning Chen
Dec 4, 2014·Metabolic Engineering·Xueyang FengHuimin Zhao
Jan 8, 2015·Frontiers in Bioengineering and Biotechnology·Ai-Qun YuMatthew Wook Chang
Feb 11, 2015·Metabolic Engineering·Brian F PflegerJens Nielsen
Apr 19, 2015·Metabolic Engineering·Robert W HaushalterJay D Keasling

❮ Previous
Next ❯

Citations

Feb 3, 2016·Microbial Cell Factories·Weihua GuoXueyang Feng
May 24, 2016·Biotechnology for Biofuels·Guokun WangShulin Chen
Oct 30, 2016·Metabolic Engineering·Charles D Rutter, Christopher V Rao
Jul 29, 2016·World Journal of Microbiology & Biotechnology·Sandy Fillet, José L Adrio
Nov 2, 2016·Biotechnology for Biofuels·Wei WangMin Zhang
Nov 20, 2016·Biotechnology and Bioengineering·Leonie WenningVerena Siewers
Dec 23, 2016·Microbial Biotechnology·Patricia Lozano-MartínezJosé Luis Revuelta
Dec 13, 2016·Biotechnology and Bioengineering·Magdalena MiklaszewskaAleksandra Królicka
May 13, 2017·Biotechnology and Bioengineering·José L Adrio
Nov 24, 2017·Frontiers in Microbiology·Shuobo Shi, Huimin Zhao
Feb 2, 2018·Critical Reviews in Biotechnology·Si-Jia XueZhen-Ming Chi
Sep 14, 2018·World Journal of Microbiology & Biotechnology·Bonnie A McNeil, David T Stuart
Nov 12, 2017·Applied Microbiology and Biotechnology·Sujit Sadashiv Jagtap, Christopher V Rao
Sep 13, 2019·Journal of Industrial Microbiology & Biotechnology·Sylwia JezierskaInge Van Bogaert
Apr 25, 2020·Biotechnology Progress·Zia FatmaHuimin Zhao
Jul 3, 2020·FEMS Yeast Research·Zhiqiang WenZongbao K Zhao
Feb 22, 2017·World Journal of Microbiology & Biotechnology·Jingyang Xu, Dehua Liu
Aug 31, 2018·Biotechnology for Biofuels·Guokun WangDongyuan Zhang
Jan 26, 2020·Biotechnology and Bioengineering·Di LiuJohn Gladden
Aug 16, 2017·Applied Microbiology and Biotechnology·Sandy FilletJosé L Adrio
Oct 28, 2017·Journal of Industrial Microbiology & Biotechnology·Bonnie A McNeil, David T Stuart
Jun 18, 2020·Metabolic Engineering Communications·Lauren T CordovaHal S Alper
Jan 26, 2021·Frontiers in Bioengineering and Biotechnology·Jaroslav HambalkoMilan Čertík
Dec 22, 2020·Frontiers in Bioengineering and Biotechnology·Anagha KrishnanDavid T Stuart
Sep 25, 2018·Current Opinion in Biotechnology·Holly EkasHal S Alper
Feb 26, 2021·Biotechnology Advances·Atrayee ChattopadhyayMrinal K Maiti
Mar 14, 2021·Journal of Industrial Microbiology & Biotechnology·Ashima Sharma, Syed Shams Yazdani
Sep 9, 2020·Journal of Agricultural and Food Chemistry·Qi GaoQiang Hua

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved