FBP WW domains and the Abl SH3 domain bind to a specific class of proline-rich ligands

The EMBO Journal
M T BedfordP Leder

Abstract

WW domains are conserved protein motifs of 38-40 amino acids found in a broad spectrum of proteins. They mediate protein-protein interactions by binding proline-rich modules in ligands. A 10 amino acid proline-rich portion of the morphogenic protein, formin, is bound in vitro by both the WW domain of the formin-binding protein 11 (FBP11) and the SH3 domain of Abl. To explore whether the FBP11 WW domain and Abl SH3 domain bind to similar ligands, we screened a mouse limb bud expression library for putative ligands of the FBP11 WW domain. In so doing, we identified eight ligands (WBP3 through WBP10), each of which contains a proline-rich region or regions. Peptide sequence comparisons of the ligands revealed a conserved motif of 10 amino acids that acts as a modular sequence binding the FBP11 WW domain, but not the WW domain of the putative signal transducing factor, hYAP65. Interestingly, the consensus ligand for the FBP11 WW domain contains residues that are also required for binding by the Abl SH3 domain. These findings support the notion that the FBP11 WW domain and the Abl SH3 domain can compete for the same proline-rich ligands and suggest that at least two subclasses of WW domains exist, namely those that bind a PPLP motif...Continue Reading

References

Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·J F GuiX D Fu
Mar 10, 1995·Cell·P Bork, B Margolis
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·J E LadburyJ Schlessinger
Sep 1, 1995·Nature Structural Biology·K M FergusonJ Schlessinger
Nov 10, 1995·The Journal of Biological Chemistry·D JungK P Campbell
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·H I Chen, M Sudol
Jan 23, 1995·FEBS Letters·K Hofmann, P Bucher
Dec 1, 1994·Trends in Biochemical Sciences·P Bork, M Sudol
Jan 1, 1994·Journal of Cell Science. Supplement·L E Marengere, T Pawson
Jan 1, 1994·Progress in Biophysics and Molecular Biology·A MusacchioM Saraste
Jul 16, 1993·Cell·D Bar-SagiJ Schlessinger
Jan 1, 1996·Molecular and Cellular Biology·X NanA Bird
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·R MaleszkaG L Miklos
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·A B SparksB K Kay
May 15, 1996·Structure·O Staub, D Rotin
Sep 20, 1996·The Journal of Biological Chemistry·D C Chan, P Leder
May 1, 1996·Trends in Biochemical Sciences·M Sudol

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Citations

Jan 29, 2011·Biochemical Genetics·Miki MatsunamiYoshinori Muto
Dec 4, 2003·Biochimica Et Biophysica Acta·Kiven E Lukong, Stéphane Richard
Nov 1, 2003·Biochemical and Biophysical Research Communications·Patricia M Bergamo FavaroSara Teresinha Olalla Saad
Dec 4, 2002·Journal of Molecular Biology·Silke WiesnerMaria J Macias
Jun 4, 1999·Progress in Biophysics and Molecular Biology·Z Songyang
Oct 3, 2002·Biochimica Et Biophysica Acta·Laura TonachiniPatrizio Castagnola
Jan 20, 1998·Trends in Genetics : TIG·C Vernet, K Artzt
Jan 29, 2002·Cellular Signalling·Jane L IlsleySteven J Winder
Feb 21, 2002·The International Journal of Biochemistry & Cell Biology·Zhen ZhangYuMin Mao
Aug 29, 2006·Nature Structural & Molecular Biology·Alexis P RideauChristopher W J Smith
Mar 18, 2000·Kidney International·O StaubD Rotin
Oct 3, 2008·The Journal of Biological Chemistry·Michael LammersAlfred Wittinghofer
Jun 2, 2009·Molecular & Cellular Proteomics : MCP·Michael KoflerChristian Freund
Feb 21, 2002·Molecular Biology of the Cell·Kathryn E Sharpless, Steven D Harris
Mar 3, 2007·Molecular Biology of the Cell·Hadiya Watson, Juan S Bonifacino
Sep 27, 2012·Science Signaling·Niall J Haslam, Denis C Shields
Aug 25, 2000·Molecular and Cellular Biology·S Yayoshi-YamamotoT Watanabe
Oct 18, 2001·Molecular and Cellular Biology·A C GoldstrohmM A Garcia-Blanco
Oct 16, 2004·Molecular and Cellular Biology·Matthew J SmithTony Pawson
Aug 2, 2005·Molecular and Cellular Biology·Robert J InghamTony Pawson
Oct 2, 2003·Bioscience, Biotechnology, and Biochemistry·Olimpia LongoPaolo Arcari
Feb 13, 2001·Biochemical and Biophysical Research Communications·F MarquésA M Genevière
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·John Karanicolas, Charles L Brooks
Jul 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·S M CartyA L Greenleaf

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