FBXO22 promotes the development of hepatocellular carcinoma by regulating the ubiquitination and degradation of p21

Journal of Experimental & Clinical Cancer Research : CR
Long ZhangXiaoping Chen

Abstract

Deregulation of ubiquitin ligases is related to the malignant progression of human cancers. F-box only protein 22 (FBXO22), an F-box E3 ligase, is a member of the F-box protein family. However, the biological function of FBXO22 in HCC and the underlying molecular mechanisms are still unclear. In this study, we explored the role of FBXO22 in HCC and its mechanism of promoting tumor development. We examined the expression of FBXO22 in normal liver cell lines, HCC cell lines, HCC tissue microarrays and fresh specimens. The correlation between FBXO22 and clinical features was analyzed in a retrospective study of 110 pairs of HCC tissue microarrays. Univariate and multivariate survival analyses were used to explore the prognostic value of FBXO22 in HCC. At the same time, the correlation between the FBXO22 and p21 was also studied in HCC samples. Knock-down and overexpression experiments, CHX and Mg132 intervention experiments, ubiquitination experiments, rescue experiments and nude mouse xenograft models were used to determine the potential mechanism by which FBXO22 promotes tumorigenesis in vitro and in vivo. The expression of FBXO22 in HCC tissues was significantly higher than in normal liver tissues. The overall survival rate and...Continue Reading

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Citations

Aug 15, 2020·Cell Death Discovery·Jiangting ChengYongchao Zhao
Aug 17, 2019·Cancers·Bahar Shamloo, Sinem Usluer
Dec 27, 2019·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Linzhi YanXueqiong Zhu
Feb 10, 2021·Cell Death Discovery·Jiangting ChengYongchao Zhao
Jun 3, 2021·International Journal of Molecular Sciences·Tingting Zou, Zhenghong Lin
Oct 3, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Si-Wei XiaShi-Zhong Zheng

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Methods Mentioned

BETA
ubiquitination
genotyping
PCR
transfection
chip
xenograft
immunoprecipitation
flow cytometry
reverse transcription-PCR
xenografts

Software Mentioned

Prism
SPSS
GraphPad
ImageJ

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