Fc gamma receptor IIb participates in maternal IgG trafficking of human placental endothelial cells

International Journal of Molecular Medicine
Tomoko IshikawaToshihiro Takizawa

Abstract

The human placental transfer of maternal IgG is crucial for fetal and newborn immunity. Low-affinity immunoglobulin gamma Fc region receptor IIb2 (FCGR2B2 or FcγRIIb2) is exclusively expressed in an IgG-containing, vesicle-like organelle (the FCGR2B2 compartment) in human placental endothelial cells; thus, we hypothesized that the FCGR2B2 compartment functions as an IgG transporter. In this study, to examine this hypothesis, we performed in vitro bio-imaging analysis of IgG trafficking by FCGR2B2 compartments using human umbilical vein endothelial cells transfected with a plasmid vector containing enhanced GFP-tagged FCGR2B2 (pFCGR2B2-EGFP). FCGR2B2-EGFP signals were detected as intracellular vesicular structures similar to FCGR2B2 compartments in vivo. The internalization and transcytosis of IgG was significantly higher in the pFCGR2B2-EGFP-transfected cells than in the mock-transfected cells, and the majority of the internalized IgG was co-localized with the FCGR2B2-EGFP signals. Furthermore, we isolated FCGR2B2 compartments from the human placenta and found that the Rab family of proteins [RAS-related protein Rab family (RABs)] were associated with FCGR2B2 compartments. Among the RABs, RAB3D was expressed predominantly in pl...Continue Reading

References

May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·R P Junghans, C L Anderson
May 20, 1999·Nature Structural Biology·K F MaxwellP M Hogarth
Oct 8, 1999·The Journal of Clinical Investigation·B L DickinsonW I Lencer
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·M B CallananD Leroux
Jun 3, 2000·Annual Review of Immunology·V Ghetie, E S Ward
Aug 1, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J M LarkinM A McNiven
Mar 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T W LydenC L Anderson
Dec 14, 2001·The Journal of Biological Chemistry·Susheela TridandapaniClark L Anderson
Aug 23, 2002·Molecular and Cellular Biology·Dietmar RiedelReinhard Jahn
Feb 5, 2003·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Jun-ichi Satoh, Yasuo Kuroda
Feb 5, 2003·The Journal of Experimental Medicine·Chaity ChaudhuryClark L Anderson
Mar 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Derry C RoopenianClark L Anderson
Jul 10, 2003·Vaccine·Neil E Simister
Jul 1, 1961·American Journal of Obstetrics and Gynecology·J DANCISP VARA
Oct 1, 1964·The Journal of Clinical Investigation·D GITLINC MORALES
Feb 10, 2004·Molecular Biology of the Cell·Steven M ClaypoolRichard S Blumberg
Mar 30, 2004·European Journal of Immunology·Dávid MedgyesiJános Gergely
Mar 3, 2005·Journal of Clinical Immunology·Toshiyuki Takai
Aug 6, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Toshihiro TakizawaJohn M Robinson
Aug 30, 2005·Immunology Letters·Reinhold E Schmidt, J Engelbert Gessner
Aug 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Bianka L GrosshansPeter Novick
Nov 18, 2006·Cell·Shigeo TakamoriReinhard Jahn
Feb 24, 2007·Molecular & Cellular Proteomics : MCP·Yannick BrunnerJean-Charles Sanchez
Aug 8, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Seyed Ali MousaviTrond Berg
Nov 16, 2007·Molecular Biology of the Cell·Bonny L DickinsonWayne I Lencer
Aug 30, 2008·Cellular and Molecular Life Sciences : CMLS·M Fukuda
Feb 24, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jonghan KimClark L Anderson
Jul 16, 2009·Nature Reviews. Molecular Cell Biology·Harald Stenmark
Dec 18, 2009·Journal of Reproductive Immunology·Sudhasri MohantyClark L Anderson
Dec 30, 2009·Molecular and Cellular Biology·Xiaolin TianJason C Mills
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sang Taek JungGeorge Georgiou
Jul 21, 2010·The Journal of Cell Biology·Lothar SchermellehHeinrich Leonhardt
Nov 17, 2010·Proteomics·Peter S McPherson
Feb 10, 2011·Journal of Cell Science·Aparna MukhopadhyayAllan W Wolkoff

❮ Previous
Next ❯

Citations

Oct 4, 2017·Seminars in Immunopathology·Madeleine F JenneweinArnaud Marchant
Jan 12, 2019·Critical Reviews in Clinical Laboratory Sciences·Eberhard Buse, Udo R Markert
Dec 1, 2017·Cell and Tissue Research·Charles Yin, Bryan Heit
Aug 26, 2016·PLoS Neglected Tropical Diseases·Zachary A KlaseRobert Malone
Jul 30, 2019·Frontiers in Immunology·Michal PyzikRichard S Blumberg
Jul 28, 2020·Frontiers in Immunology·Sibel KaraAnja Lux

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
PCR
enzyme-linked immunosorbent assay
electrophoresis
immunoprecipitation
ELISA
electron microscopy
gene-knockout
GTPases

Software Mentioned

FCGRT
SPSS
DNASIS
MetaMorph
siDirect
Mascot Search
DeCyder 2D Differential Analysis
Photoshop CS
Multi Gauge

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.