Fc receptor-mediated, antibody-dependent enhancement of bacteriophage lambda-mediated gene transfer in mammalian cells

Virology
Ramil SapinoroStephen Dewhurst

Abstract

Lambda phage vectors mediate gene transfer in cultured mammalian cells and in live mice, and in vivo phage-mediated gene expression is increased when mice are pre-immunized with bacteriophage lambda. We now show that, like eukaryotic viruses, bacteriophage vectors are subject to Fc receptor-mediated, antibody-dependent enhancement of infection in mammalian cells. Antibody-dependent enhancement of phage gene transfer required FcgammaRI, but not its associated gamma-chain, and was not supported by other FcgammaR family members (FcgammaRIIA, FcgammaRIIB, and FcgammaRIII). Studies using chlorpromazine and latrunculin A revealed an important role for clathrin-mediated endocytosis (chlorpromazine) and actin filaments (latrunculin A) in antibody-enhanced phage gene transfer. This was confirmed by experiments using inhibitors of endosomal acidification (bafilomycin A1, monensin) and by immunocytochemical colocalization of internalized phage particles with early endosome-associated protein-1 (EAA1). In contrast, microtubule-targeting agents (nocodazole, taxol) increased the efficiency of antibody-enhanced phage gene transfer. These results reveal an unexpected antibody-dependent, FcgammaRI-mediated enhancement of phage transduction in m...Continue Reading

References

Nov 1, 1991·The Journal of Clinical Investigation·Z IndikA D Schreiber
May 7, 1990·Biochimica Et Biophysica Acta·H H MollenhauerL D Rowe
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E J BowmanK Altendorf
Jan 1, 1969·Advances in Virus Research·J D Almeida, A P Waterson
Mar 1, 1972·Virology·M R Geier, C R Merril
Oct 8, 1971·Nature·C R MerrilJ C Petricciani
Jun 1, 1983·The Journal of Cell Biology·J HarfordR D Klausner
Apr 16, 1981·Nature·J S Porterfield
Jul 1, 1996·Journal of Virological Methods·S P ForestellR J Rigg
Jan 1, 1997·Annual Review of Immunology·M Daëron
Feb 17, 1999·Biochimica Et Biophysica Acta·V IvanenkovA G Menon
Mar 30, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D LaroccaA Baird
Nov 2, 1999·Biochemical and Biophysical Research Communications·P D KassnerD Larocca
Mar 13, 2001·Annual Review of Immunology·J V Ravetch, S Bolland
Apr 11, 2001·Advances in Immunology·P W Parren, D R Burton
May 11, 2001·The Journal of Biological Chemistry·A EguchiM Nakanishi
Jun 18, 2003·The Journal of General Virology·M J WallaceP C McMinn
Nov 20, 2003·Reviews in Medical Virology·Ayato Takada, Yoshihiro Kawaoka
Jan 22, 2004·FEMS Immunology and Medical Microbiology·Jason R Clark, John B March
Jun 15, 2004·Vaccine·Catherine D Jepson, John B March
Oct 15, 2005·Nucleic Acids Research·Christine N ZanghiStephen Dewhurst
Aug 22, 2006·Journal of Leukocyte Biology·Zhen-Yu HuangAlan D Schreiber

❮ Previous
Next ❯

Citations

Jan 21, 2014·Applied Microbiology and Biotechnology·Jessica NicastroRoderick A Slavcev
Mar 25, 2009·Infection and Immunity·Xiangyun HeHanping Feng
May 13, 2010·Genetic Vaccines and Therapy·Amir GhaemiFarzin Roohvand
Mar 2, 2013·PloS One·Leticia V BentancorMarina S Palermo
Jun 24, 2014·Expert Opinion on Drug Delivery·Babak Bakhshinejad, Majid Sadeghizadeh
Oct 31, 2016·Advanced Drug Delivery Reviews·Mahdi KarimiMichael R Hamblin
Dec 15, 2015·PloS One·Tetyana DashivetsTilman Schlothauer
Nov 16, 2017·Immunology·Kelly B Arnold, Amy W Chung
Jan 13, 2009·Blood·Michelle M CollazoWilliam G Kerr
Oct 1, 2016·Oncotarget·María Del Mar Valenzuela-MembrivesMaría Esther Fárez-Vidal
Nov 28, 2020·Trends in Microbiology·Katie BodnerMarkus W Covert
May 21, 2021·Annual Review of Virology·Medeea PopescuPaul L Bollyky

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antibody-Dependent Enhancement

Antibody-dependent enhancement of viral infection is the entry of virus into host cells mediated by antiviral antibodies interacting with Fc or complement receptors. This has been most extensively observed with the dengue virus. Find the latest research on antibody-dependent enhancement here.

Antipsychotic Drugs

Antipsychotic drugs are a class of medication primarily used to manage psychosis (including delusions, hallucinations, paranoia or disordered thought), principally in schizophrenia and bipolar disorder. Discover the latest research on antipsychotic drugs here

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.