FcgammaRI (CD64) resides constitutively in lipid rafts

Immunology Letters
Jeffrey M BeekmanJeanette H W Leusen

Abstract

Cellular membranes contain microdomains known as 'lipid rafts' or detergent-insoluble microdomains (DRM), enriched in cholesterol and sphingolipids. DRM can play an important role in many cellular processes, including signal transduction, cytoskeletal organization, and pathogen entry. Many receptors like T cell receptors, B cell receptors and IgE receptors have been shown to reside in DRM. The majority of these receptors depend on multivalent ligand interaction to associate with these microdomains. We, here, study association between the high affinity IgG receptor, FcgammaRI (CD64), and membrane microdomains. FcgammaRI is a 72kDa type I glycoprotein that can mediate phagocytosis of opsonized pathogens, but can also effectively capture small immune complexes, and facilitates antigen presentation. We found FcgammaRI to predominantly reside within detergent-insoluble buoyant membranes, together with FcRgamma-chain, but independent of cross-linking ligand. With the use of confocal imaging, FcgammaRI was found to co-patch with GM1, a microdomain-enriched glycolipid. Depletion of cellular cholesterol, furthermore, modulated FcgammaRI-ligand interactions. These data indicated FcgammaRI to reside within lipid rafts without prior trigge...Continue Reading

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Citations

Apr 17, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ayman El-Sayed, Hideyoshi Harashima
Mar 13, 2014·Nature Immunology·Jelena S BezbradicaRuslan Medzhitov
May 1, 2015·Nature Communications·Masato KiyoshiKouhei Tsumoto
Jun 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stylianos BournazosIan Dransfield
Mar 1, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julio M RubioJesús Balsinde
Aug 9, 2018·Frontiers in Immunology·Julio M RubioJesús Balsinde
Jul 26, 2018·Science Signaling·Arianne M BrandsmaJeanette H W Leusen
Jul 28, 2020·Frontiers in Immunology·Sibel KaraAnja Lux
Jan 13, 2021·Proceedings of the National Academy of Sciences of the United States of America·David C MontefioriPeter S Kim

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