Fck1, a C-type cyclin-dependent kinase, interacts with Fcc1 to regulate development and secondary metabolism in Fusarium verticillioides

Fungal Genetics and Biology : FG & B
B H Bluhm, Charles Woloshuk

Abstract

In Fusarium verticillioides, the C-type cyclin Fcc1 is a global regulator of gene expression. In Saccharomyces cerevisiae and other organisms, C-type cyclins regulate the activity of specific cyclin-dependent kinases through physical association. We identified FCK1, a gene encoding a cyclin-dependent kinase in F. verticillioides. The Fck1 protein is predicted to contain a cyclin-binding motif and a serine-threonine protein kinase domain homologous to previously described cyclin-dependent kinases. Disruption of FCK1 resulted in pleiotropic morphological defects including reduced growth, conidiation, fumonisin B(1) (FB(1)) production, and enhanced pigmentation. Two-hybrid analysis indicated a strong physical interaction between Fcc1 and Fck1. This study presents the first description of the interaction between a C-type cyclin and a cyclin-dependent kinase in a filamentous fungus and provides new insight regarding a molecular mechanism that regulates aspects of maize kernel colonization by F. verticillioides.

References

Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·S KuchinM Carlson
Mar 9, 1995·Nature·S M LiaoR A Young
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Aug 7, 1999·FEMS Microbiology Letters·W B Shim, C P Woloshuk
May 11, 2000·Molecular and Cellular Biology·J R RohdeI Sadowski
Feb 18, 2003·Molecular and Cellular Biology·Mainul HoqueTsafi Pe'ery
Sep 6, 2003·Applied and Environmental Microbiology·Joseph E FlahertyCharles P Woloshuk
Oct 24, 2003·Current Genetics·Hiroshi Mitsuzawa, Akira Ishihama
May 7, 2004·Applied and Environmental Microbiology·Joseph E Flaherty, Charles P Woloshuk
Oct 13, 2004·Nucleic Acids Research·Benjamin GuglielmiMichel Werner
Feb 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aseem Z AnsariMark Ptashne

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Citations

Apr 23, 2009·Journal of Agricultural and Food Chemistry·Kyung MyungAna M Calvo
Apr 7, 2010·Critical Reviews in Microbiology·Adeline PicotFlorence Richard-Forget
Jun 21, 2012·Fungal Genetics and Biology : FG & B·Robert A E ButchkoPhilipp Wiemann
Oct 20, 2012·FEMS Microbiology Reviews·Charles P Woloshuk, Won-Bo Shim
Apr 25, 2015·Journal of Agricultural and Food Chemistry·Davide FerrigoRoberto Causin
Nov 17, 2009·Fungal Genetics and Biology : FG & B·Xiaoying ZhouJin-Rong Xu

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