Fe2+ binds iron responsive element-RNA, selectively changing protein-binding affinities and regulating mRNA repression and activation.

Proceedings of the National Academy of Sciences of the United States of America
Jia MaDixie J Goss

Abstract

Iron increases synthesis rates of proteins encoded in iron-responsive element (IRE)-mRNAs; metabolic iron ("free," "labile") is Fe(2+). The noncoding IRE-RNA structure, approximately 30 nt, folds into a stem loop to control synthesis of proteins in iron trafficking, cell cycling, and nervous system function. IRE-RNA riboregulators bind specifically to iron-regulatory proteins (IRP) proteins, inhibiting ribosome binding. Deletion of the IRE-RNA from an mRNA decreases both IRP binding and IRP-independent protein synthesis, indicating effects of other "factors." Current models of IRE-mRNA regulation, emphasizing iron-dependent degradation/modification of IRP, lack answers about how iron increases IRE-RNA/IRP protein dissociation or how IRE-RNA, after IRP dissociation, influences protein synthesis rates. However, we observed Fe(2+) (anaerobic) or Mn(2+) selectively increase the IRE-RNA/IRP K(D). Here we show: (i) Fe(2+) binds to the IRE-RNA, altering its conformation (by 2-aminopurine fluorescence and ethidium bromide displacement); (ii) metal ions increase translation of IRE-mRNA in vitro; (iii) eukaryotic initiation factor (eIF)4F binds specifically with high affinity to IRE-RNA; (iv) Fe(2+) increased eIF4F/IRE-RNA binding, which...Continue Reading

References

Mar 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J ZähringerH N Munro
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C M HarrellE C Theil
Oct 13, 1995·The Journal of Biological Chemistry·W BreuerZ I Cabantchik
May 15, 1997·Analytical Biochemistry·S EpsztejnI Cabantchik
Nov 4, 2000·The Journal of Biological Chemistry·E C Theil, R S Eisenstein
Jun 15, 2005·Annual Review of Biochemistry·Deborah C JohnsonMichael K Johnson
Jul 20, 2006·Nature Chemical Biology·Tracey A Rouault
Jul 29, 2006·Biochimica Et Biophysica Acta·Michelle L WallanderRichard S Eisenstein
Jan 24, 2007·Molecular and Cellular Biology·Jian WangKostas Pantopoulos
Apr 10, 2007·Nature Structural & Molecular Biology·Mayka SanchezMatthias W Hentze
May 22, 2007·RNA·Paul Piccinelli, Tore Samuelsson
Sep 13, 2007·Cellular and Molecular Life Sciences : CMLS·R Leipuviene, E C Theil
May 21, 2008·Annual Review of Nutrition·Martina U MuckenthalerMatthias W Hentze
Aug 5, 2008·The Journal of Biological Chemistry·Camila O dos SantosMitchell J Weiss
Sep 2, 2009·The Journal of Biological Chemistry·Mateen A KhanElizabeth C Theil
Sep 19, 2009·Science·Ajay A VashishtJames A Wohlschlegel
Oct 15, 2009·Chemical Reviews·Elizabeth C Theil, Dixie J Goss
Jan 23, 2010·Nature Reviews. Molecular Cell Biology·Richard J JacksonTatyana V Pestova
May 7, 2010·The Journal of Biological Chemistry·William C Merrick
Jul 7, 2010·Cell·Matthias W HentzeClara Camaschella
Jul 20, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Robert C Hider, Xiao L Kong
Jan 25, 2012·RNA·Alina DabaKostas Pantopoulos

❮ Previous
Next ❯

Citations

Oct 10, 2013·Inorganic Chemistry·Elizabeth C Theil
May 23, 2013·Nature Chemistry·Chiaolong HsiaoLoren Dean Williams
Jan 5, 2016·Trends in Biochemical Sciences·Alexander R BogdanYoshiaki Tsuji
Mar 10, 2015·Biochimie·Christopher S Fraser
Jun 9, 2014·Plant Science : an International Journal of Experimental Plant Biology·Takanori Kobayashi, Naoko K Nishizawa
May 3, 2016·Accounts of Chemical Research·Elizabeth C TheilRabindra K Behera
Sep 12, 2014·Metallomics : Integrated Biometal Science·Elizabeth C Theil
Apr 16, 2014·Chemical Reviews·W Luke WardVictoria J DeRose
Oct 30, 2015·Radiation and Environmental Biophysics·Li-hua XieHai-qian Zhang
Aug 19, 2020·Antioxidants & Redox Signaling·Behrouz HassanniaTom Vanden Berghe
Jul 20, 2018·The Journal of Biological Chemistry·Jessica A ZinskieNatalia Shcherbik
Sep 11, 2020·Plant Molecular Biology·Takeshi SenouraNaoko K Nishizawa
Jun 17, 2016·RNA·Dhwani N Rupani, Gregory J Connell
Apr 25, 2020·Frontiers in Oncology·Rikki A M BrownPeter J Leedman
Oct 9, 2020·Chemical Communications : Chem Comm·Maria Inês P S LeitãoAna Petronilho
Aug 24, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Mateen A KhanJaved M Khan
May 7, 2021·Chemical Society Reviews·Dmytro DziubaYves Mély
Sep 20, 2017·Chemical Research in Toxicology·Ke BianDeyu Li
Mar 10, 2015·Inorganic Chemistry·Stephanie A SanderJanet R Morrow
Aug 18, 2020·ACS Chemical Biology·Herschel MukherjeeJennifer C Petter
Oct 14, 2021·Journal of Radiological Protection : Official Journal of the Society for Radiological Protection·Lisa M ManglassNicole E Martinez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.