Feasibility of Electroporation in Bone and in the Surrounding Clinically Relevant Structures: A Preclinical Investigation

Technology in Cancer Research & Treatment
Matilde TschonMilena Fini

Abstract

Skeletal metastases are a common cause of severe morbidity, reduction in quality of life and often early mortality. Consequently, improvements in therapies are necessary. Electroporation uses electric energy to alter cancer cell membrane permeability and enhance the local uptake of chemotherapeutics, thus leading to local tumor control. The aim of this study was to investigate the feasibility and safety of delivering electric field protocols causing electroporation of healthy bone and structures of clinical relevance using small and large animal models. Reversible electroporation was used in the rabbit sciatic nerve by applying 2 series of 8 pulses 100ms long at 1000 V/cm. Irreversible electroporation was used in rabbit distal femur condyles and in sheep vertebral body by applying 120 pulses 100ms long at 1750 V/cm. Any effect on surrounding sensitive structures was investigated. Reversible electroporation of sciatic nerve was associated with transient foot functional deficit that completely recovered at 30 days. Irreversible electroporation removed cells from trabeculae in the femurs of rabbits and in the vertebral body of sheep. After irreversible protocol, histology and microtomography demonstrated that the trabecular struct...Continue Reading

References

Jan 11, 1991·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·L M MirC Paoletti
Dec 1, 1987·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A M ParfittR R Recker
Jan 25, 2000·The Journal of Surgical Research·L MartiniR Giardino
Jun 1, 2000·Anti-cancer Drugs·M J JaroszeskiR Heller
Jun 6, 2000·Advanced Drug Delivery Reviews·L M Mir, S Orlowski
Mar 5, 2003·Technology in Cancer Research & Treatment·Dietmar P RabussayPaul M Goldfarb
Sep 4, 2004·Clinical Orthopaedics and Related Research·Kenichi IsobeKunio Takaoka
Jan 24, 2007·Technology in Cancer Research & Treatment·Boris RubinskyPaul Mikus
Jan 11, 2008·Nature·Sohail F TavazoieJoan Massagué
May 24, 2008·Annals of Surgical Oncology·P QuaglinoM G Bernengo
Jun 3, 2009·The Journal of Bone and Joint Surgery. American Volume·Jacob BickelsZvi Lidar
Oct 31, 2009·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Sun-Ren ShengYi-Fei Zhou
Sep 8, 2010·Journal of Biomechanical Engineering·Mary PhillipsBoris Rubinsky
Nov 3, 2010·The Journal of Bone and Joint Surgery. British Volume·M FiniR Cadossi
Mar 29, 2011·Journal of Vascular and Interventional Radiology : JVIR·Kenneth R ThomsonAndrew Haydon
Nov 28, 2012·Medical & Biological Engineering & Computing·D MiklavčičT Jarm
Jul 9, 2013·Clinical & Experimental Metastasis·Milena FiniVeronica Borsari
Dec 3, 2013·BMC Cancer·Corrado CaracòPaolo Antonio Ascierto
Mar 25, 2014·Journal of Vascular and Interventional Radiology : JVIR·Hester J SchefferMartijn R Meijerink
May 9, 2014·Trends in Endocrinology and Metabolism : TEM·Gillian BrowneJane B Lian
Sep 13, 2014·Cardiovascular and Interventional Radiology·Govindarajan NarayananJose Yrizarry
Mar 20, 2015·BMJ : British Medical Journal·Mohammad Al EfishatMatthew J Weiss
Jun 2, 2015·Radiology and Oncology·Roberto GirelliBassi Claudio

❮ Previous
Next ❯

Citations

Nov 16, 2017·Biomedical Microdevices·Wei ChaiLizhi Niu
Mar 5, 2016·The American Journal of Hospice & Palliative Care·Marco CascellaArturo Cuomo
May 16, 2018·Technology in Cancer Research & Treatment·Helena CindričDamijan Miklavčič
Dec 18, 2020·Frontiers in Bioengineering and Biotechnology·Benjamin GantenbeinDevina Purmessur
Jun 3, 2021·International Journal of Environmental Research and Public Health·Vincenza GranataFrancesco Izzo

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
Fluorescence imaging
Fluorescence
delamination

Software Mentioned

Axio Vision
Skyscan
SPSS Statistics
COMSOL Multiphysics
NRecon

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.