Feasibility of gamma irradiation as a stabilisation technique in the preparation of tissue reference materials for a range of shellfish toxins

Analytical and Bioanalytical Chemistry
Pearse McCarronPhilipp Hess

Abstract

The effect of gamma-irradiation on concentrations of hydrophilic and lipophilic phycotoxins has been investigated by use of HPLC-UV and LC-MS. Pure toxins in organic solvents and toxins in mussel (Mytilus edulis) tissues were irradiated at three different doses. In solution all toxin concentrations were reduced to some extent. Most severe decreases were observed for domoic acid and yessotoxin, for which the smallest dose of irradiation led to almost complete destruction. For pectenotoxin-2 the decrease in concentration was less severe but still continuous with increasing dose. Azaspiracid-1 and okadaic acid were the least affected in solution. In shellfish tissue the decrease in toxin concentrations was much reduced compared with the effect in solution. After irradiation at the highest dose reductions in concentrations were between ca. 5 and 20% for the lipophilic toxins and there was no statistical difference between control and irradiated samples for azaspiracids in tissue. Irradiation of shellfish tissues contaminated with domoic acid led to a more continuous decrease in the amount of the toxin with increasing dose. The effect of irradiation on the viability of microbial activity in shellfish tissues was assessed by using to...Continue Reading

References

Jan 28, 2004·Analytical and Bioanalytical Chemistry·Vincent Clancy, Scott Willie
Feb 21, 2006·Toxicon : Official Journal of the International Society on Toxinology·P McCarron, Philipp Hess

Citations

Sep 28, 2006·Analytical and Bioanalytical Chemistry·Philipp HessMichael A Quilliam
Jan 27, 2007·Analytical and Bioanalytical Chemistry·Pearse McCarronPhilipp Hess
Mar 19, 2011·Analytical and Bioanalytical Chemistry·Pearse McCarronPhilipp Hess
Oct 12, 2011·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·A P LouppisM G Kontominas
Aug 3, 2014·Toxicon : Official Journal of the International Society on Toxinology·Andrew TurnerStephen Burrell
Jun 5, 2015·Critical Reviews in Analytical Chemistry·Anna KiełbasaBogusław Buszewski
Oct 23, 2014·Analytical and Bioanalytical Chemistry·P McCarronMichael A Quilliam
Jun 26, 2013·Toxicon : Official Journal of the International Society on Toxinology·Andrew TurnerWendy A Higman
Nov 11, 2016·Analytical and Bioanalytical Chemistry·Pearse McCarronMichael A Quilliam
Jan 30, 2020·Foods·Antía Martinez-AlboresAlberto Cepeda

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