Feasibility of Mass-Spectrometry to Lower Cost and Blood Volume Requirements for Assessment of B Vitamins in Patients Undergoing Bariatric Surgery

Metabolites
Seth ArmahNana Gletsu-Miller

Abstract

Bariatric surgery induces deficiencies in a combination of B vitamins. However, high costs and a large blood volume requirement are barriers to routine screening. We adapted and validated a method coupling tandem mass spectrometry (MS/MS) with high-performance liquid chromatography (HPLC) to facilitate cost-effective analysis for simultaneous detection of B vitamins in low volumes of plasma. Based on existing methods, pooled plasma was extracted using hexane and acetonitrile and seven B vitamin analytes were separated using HPLC. Detection was performed with an Agilent 6460 triple quadrupole tandem mass spectrometer (MS/MS) using electrospray ionization in the positive ion mode. We evaluated linearity, recovery, precision, and limit of detection, as well as costs of the assay. We evaluated seven B vitamins from plasma; five (riboflavin, nicotinamide, pantothenic acid, pyridoxine, and biotin) were detected and quantified with precision and linearity. Recovery ranged from 63 to 81% for each of the vitamins, except for nicotinamide-the recovery of which was suppressed to 40%, due to plasma matrix effects. We demonstrated the feasibility of the HPLC-MS/MS method for use in patients who undergo bariatric surgery by analyzing pooled ...Continue Reading

References

Feb 5, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yong WangGuo-An Luo
Aug 13, 2011·Current Opinion in Clinical Nutrition and Metabolic Care·Yvonne Lamers
Aug 31, 2011·International Journal of Obesity : Journal of the International Association for the Study of Obesity·N Gletsu-MillerT R Ziegler
Jul 3, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tristan E LipkieMario G Ferruzzi
Sep 17, 2013·Advances in Nutrition·Nana Gletsu-Miller, Breanne N Wright
Oct 27, 2015·Current Neurology and Neuroscience Reports·Jerry Clay Goodman
Jan 26, 2016·Surgery for Obesity and Related Diseases : Official Journal of the American Society for Bariatric Surgery·Renee A MischlerNana Gletsu-Miller
Dec 15, 2016·European Journal of Clinical Nutrition·I BazuinA K Boer
Apr 19, 2017·Surgery for Obesity and Related Diseases : Official Journal of the American Society for Bariatric Surgery·Tair Ben-PoratRam Weiss
Aug 29, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·R J A C Roelofsen-de BeerY B de Rijke
Oct 22, 2017·Surgery for Obesity and Related Diseases : Official Journal of the American Society for Bariatric Surgery·M CaronLaurent Biertho
Jul 30, 2018·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiaoqin ChengChunjiu Zhong
Mar 8, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Min ZhangXiaoDai Cui

❮ Previous
Next ❯

Citations

Jan 20, 2021·Scandinavian Journal of Clinical and Laboratory Investigation·Allan WeimannEmil L Larsen
Oct 6, 2021·Nature Communications·Florian Leonardus Rudolfus LucasGiovanni Maglia

❮ Previous
Next ❯

Methods Mentioned

BETA
blood draw
reverse-phase chromatography

Software Mentioned

Excel
Agilent Mass Hunter

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.