Feasibility of microRNA profiling in human inner ear perilymph

Neuroreport
Matthew ShewHinrich Staecker

Abstract

Hearing loss is common and caused by a wide range of molecular and cellular pathologies. Current diagnosis of hearing loss depends on a combination of physiologic testing, patient history, and in some cases genetic testing. Currently, no biopsy or equivalent procedure exists to diagnose inner ear disorders. MicroRNAs (miRNA) are short ribonucleic acids that regulate a variety of cellular processes. They have been found to be reliable markers for a variety of disease processes. In particular, a variety of miRNAs that are markers for neurodegenerative disease have been identified in cerebrospinal fluid. The aim of this study was to determine whether miRNAs could be identified in human perilymph potentially leading to the development of biomarkers for inner ear disease. Prospective sampling of human perilymph and its analysis were carried out. Patients undergoing surgery in which the inner ear is opened as part of the procedure (cochlear implantation, stapedectomy, labyrinthectomy) were recruited. A total of 2-5 μl of perilymph was collected and analyzed using Affymetrix GeneChip miRNA 4.0 microarrays. MiRNA common to all sampling approaches were selected. Analysis of miRNAs was carried out by evaluating miRNA targets in a cochlea...Continue Reading

References

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Citations

Jun 12, 2019·PloS One·Hsiao-Chun LinKonstantina M Stankovic
Jul 15, 2020·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Matthew ShewHinrich Staecker
Oct 22, 2019·Frontiers in Neurology·Lukas D LandeggerKonstantina M Stankovic
Jun 14, 2019·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Helena WichovaHinrich Staecker
Jul 19, 2019·Hearing Research·Anne G M SchilderHelen Blackshaw
Sep 8, 2019·Hearing Research·Krishna BommakantiKonstantina M Stankovic
Jul 6, 2021·Frontiers in Neurology·Matthew ShewHinrich Staecker
Aug 6, 2021·Laryngo- rhino- otologie·Athanasia Warnecke, Anja Giesemann
May 12, 2021·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Matthew ShewHinrich Staecker
Oct 8, 2021·Neuroscience Letters·Pei ZhuangHinrich Staecker

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Methods Mentioned

BETA
chip
cochlear implant
biopsy

Software Mentioned

IPA
Affymetrix Expression Console

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