PMID: 9180913Aug 1, 1997Paper

Feasibility of peripheral blood stem cell (PBSC) and peripheral blood mononuclear cell (PBMNC) separation in children with a body weight below 20 KG

Medical and Pediatric Oncology
C UrbanS Burdach

Abstract

Nine children from 10 to 76 months (median 28.0), weighing 8.5 to 19.7 kg (median 13.0 kg) underwent peripheral blood stem cell separation (PBSCS) or peripheral blood mononuclear cell separation (PBMNCS), after insertion of a double-lumen central venous catheter (8-10 French). Separations were performed with a continuous flow blood separator (Fen-wall CS 3000 plus), running a specially adopted separation-program. In 7 children (5 with neuroblastoma IV, 1 with multifocal Ewing's sarcoma, and 1 with rhabdomyosarcoma IV), stem cells were mobilized by application of G-CSF at a dosage of 15-27.7 micrograms/kg body weight (median 16.25) subcutaneously following high-dose chemotherapy, according to the disease-related protocols, whereas 2 children had PBMNCS to induce graft vs. leukemia (GvL)-reaction in the HLA-identical sibling suffering from relapsed chronic myelogenous leukemia (CML: n = 1), or chronic myelomonocytic leukemia (CMML: n = 1) after allogeneic BMT. In all cases, the collecting procedure was performed after filling the cell separator with priming solution consisting of 2 U of irradiated and washed packed red cells, 250 ml human albumin, and 0.9% NaCl. In the 7 patients with solid tumors between 0.45 and 62.7 x 10(6) CD...Continue Reading

References

Apr 1, 1992·Pediatric Hematology and Oncology·I Slavc, C Urban
May 1, 1993·The American Journal of Pediatric Hematology/oncology·J W Singer

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Citations

Jul 17, 1999·Pediatric Hematology and Oncology·M Kalmanti, E Stiakaki
Aug 31, 2002·Journal of Hematotherapy & Stem Cell Research·Julián SevillaMiguel Angel Díaz
Nov 24, 2011·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Dobrila VeljkovicEmilija Lazic
Jan 6, 2015·Journal of Clinical Apheresis·Rosario Salazar-RiojasCésar Homero Gutiérrez-Aguirre

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