Abstract
Nine children from 10 to 76 months (median 28.0), weighing 8.5 to 19.7 kg (median 13.0 kg) underwent peripheral blood stem cell separation (PBSCS) or peripheral blood mononuclear cell separation (PBMNCS), after insertion of a double-lumen central venous catheter (8-10 French). Separations were performed with a continuous flow blood separator (Fen-wall CS 3000 plus), running a specially adopted separation-program. In 7 children (5 with neuroblastoma IV, 1 with multifocal Ewing's sarcoma, and 1 with rhabdomyosarcoma IV), stem cells were mobilized by application of G-CSF at a dosage of 15-27.7 micrograms/kg body weight (median 16.25) subcutaneously following high-dose chemotherapy, according to the disease-related protocols, whereas 2 children had PBMNCS to induce graft vs. leukemia (GvL)-reaction in the HLA-identical sibling suffering from relapsed chronic myelogenous leukemia (CML: n = 1), or chronic myelomonocytic leukemia (CMML: n = 1) after allogeneic BMT. In all cases, the collecting procedure was performed after filling the cell separator with priming solution consisting of 2 U of irradiated and washed packed red cells, 250 ml human albumin, and 0.9% NaCl. In the 7 patients with solid tumors between 0.45 and 62.7 x 10(6) CD...Continue Reading
References
Apr 1, 1992·Pediatric Hematology and Oncology·I Slavc, C Urban
Jun 20, 1991·The New England Journal of Medicine·J NemunaitisM Gold
May 1, 1993·The American Journal of Pediatric Hematology/oncology·J W Singer
Jan 1, 1995·Pediatric Hematology and Oncology·W SchwingerO A Haas
Citations
Feb 27, 2001·Bone Marrow Transplantation·M A DíazF Deméocq
Feb 9, 2002·Journal of Clinical Apheresis·V WittH Gadner
Mar 7, 2003·Bone Marrow Transplantation·D OrbachJ Michon
Jul 17, 1999·Pediatric Hematology and Oncology·M Kalmanti, E Stiakaki
Aug 31, 2002·Journal of Hematotherapy & Stem Cell Research·Julián SevillaMiguel Angel Díaz
May 14, 2005·Journal of Clinical Apheresis·Hyun-Jung ChoDae-Won Kim
Jul 22, 2005·Journal of Clinical Apheresis·Fernando RavagnaniRoberto Luksch
Dec 15, 2007·Journal of Clinical Apheresis·Volker WittGerhard Fritsch
Jul 17, 1999·Pediatric Hematology and Oncology·I DolgopolovG Mentkevich
May 20, 1998·The Tohoku Journal of Experimental Medicine·M YoshinariK Iinuma
Nov 24, 2011·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Dobrila VeljkovicEmilija Lazic
Jan 6, 2015·Journal of Clinical Apheresis·Rosario Salazar-RiojasCésar Homero Gutiérrez-Aguirre
May 18, 1999·The Tohoku Journal of Experimental Medicine·A SatoK Iinuma
Jun 19, 2012·Bone Marrow Transplantation·S M van WalravenL M Ball
Feb 14, 2004·Transfusion·Charles D BolanSusan F Leitman