Feasibility of supercritical fluid chromatography/mass spectrometry of polypeptides with up to 40-mers

Analytical Chemistry
J ZhengL T Taylor

Abstract

Supercritical fluid chromatography (SFC) provides a number of advantages over traditional HPLC such as speed, practical use of longer columns, a normal-phase retention mechanism, and reduced use of organic solvents. Yet, it has been a technique traditionally limited to relatively nonpolar compounds. The nature of SFC mobile and stationary phases did not allow the elution of ionic compounds or of peptides, except, in the latter case, for the most hydrophobic peptides. The characterization of peptides is critically important for drug discovery and development in the pharmaceutical industry, as well as for a variety of other important applications. Here, for the first time to our knowledge, we show that relatively large peptides (at least 40 mers), containing a variety of acidic and basic residues, can be eluted in SFC. We used trifluoroacetic acid as additive in a CO2/methanol mobile phase to suppress deprotonation of peptide carboxylic acid groups and to protonate peptide amino groups. A 2-ethylpyridine bonded silica column, which was specifically developed for SFC, was used for the majority of this work. The relatively simple mobile phase was compatible with mass spectrometric detection.

References

Oct 22, 2002·Journal of the American Society for Mass Spectrometry·J David PinkstonB David Clair
Sep 4, 2004·Journal of Biochemical and Biophysical Methods·Tatsunari Yoshida
Nov 25, 2004·Current Opinion in Biotechnology·Knut AdermannWolf-Georg Forssmann
Dec 4, 2004·Analytical and Bioanalytical Chemistry·Harald John

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Citations

Nov 19, 2010·Bioanalysis·David TognarelliWalt Caldwell
Jan 16, 2013·Journal of Bioscience and Bioengineering·Muneo Saito
Sep 1, 2015·Analytica Chimica Acta·Charlene GaleaYvan Vander Heyden
Apr 14, 2015·Journal of Pharmaceutical and Biomedical Analysis·Ann Van Eeckhaut, Debby Mangelings
Jul 22, 2014·Journal of Pharmaceutical and Biomedical Analysis·Matthias D'HondtBart De Spiegeleer
Jan 22, 2015·Journal of Chromatography. a·Eric Lesellier, Caroline West
Jun 28, 2017·Journal of Chromatography. a·David SpeybrouckDavid Corens
Dec 1, 2006·Journal of Mass Spectrometry : JMS
Mar 28, 2008·Journal of Separation Science·Eric AbbottHaleem J Issaq
Mar 28, 2008·Journal of Separation Science·Fangbiao Li, Yunsheng Hsieh
Nov 12, 2020·Journal of Pharmaceutical and Biomedical Analysis·Jérémy MolineauCaroline West
May 1, 2020·Journal of Pharmaceutical and Biomedical Analysis·Manuel Ventura
Aug 2, 2017·Journal of Chromatography. a·Raffeal Bennett, Susan V Olesik
Apr 23, 2008·Analytical Chemistry·Larry T Taylor
Mar 23, 2019·The Journal of Organic Chemistry·Albert Isidro-LlobetFrank Roschangar

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