Feasibility of up-regulating CD4(+)CD25(+) Tregs by IFN-γ in myasthenia gravis patients

BMC Neurology
Shuo HuangLi-Jun Chi

Abstract

In myasthenia gravis (MG) patients, the dysfunction of CD4(+)CD25(+) regulatory T cells (CD4(+)CD25(+) Tregs) may be one of the important pathogenesis of MG. Currently, the role of IFN-γ in autoimmune diseases is still controversial and needs further exploration. In this study, whether IFN-γ can induce CD4(+)CD25(-) T cells into CD4(+)CD25(+) Tregs in MG in vitro was investigated systematically. Flow cytometry was used to analyze the number of CD4(+)CD25(+) Tregs in MG patients and healthy controls (HCs). CD4(+)CD25(-) T cells were separated from the peripheral blood mononuclear cells of MG patients and HCs, and the CD4(+)CD25(+) Tregs were separated from HCs by Magnetic cell sorting (MACS). IFN-γ with different concentrations was used to stimulate CD4(+)CD25(-) T cells. The percentages of the induced CD4(+)CD25(+) T cells were detected by flow cytometry. The FoxP3 expression of the induced CD4(+)CD25(+) T cells in MG patients was detected by real-time PCR at mRNA level. The induced CD4(+)CD25(+) T cells were co-cultured with autologous CD4(+)CD25(-) T cells to estimate the suppressive ability of the induced CD4(+)CD25(+) T cells to CD4(+)CD25(-) T cells. It shows the percentages of CD4(+)CD25(+) T cells among CD4(+) T cells ha...Continue Reading

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Citations

Feb 7, 2018·Annals of the New York Academy of Sciences·Jose Adolfo VillegasNadine Dragin
May 25, 2021·Seminars in Cell & Developmental Biology·Verena Fischer, Melanie Haffner-Luntzer
Jul 16, 2021·Cardiovascular Research·George Markousis-MavrogenisPeter van der Meer

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Methods Mentioned

BETA
flow cytometry
FACS
PCR

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