Feasibility of using urinary N7-(2-carbamoyl-2-hydroxyethyl) Guanine as a biomarker for acrylamide exposed workers

Journal of Exposure Science & Environmental Epidemiology
Yu-Fang HuangKuen-Yuh Wu

Abstract

Acrylamide (AA), a probable human carcinogen, is a widely-used industrial chemical but is also present in tobacco smoke and carbohydrate-rich foods processed at high temperatures. AA is metabolized to glycidamide (GA) to cause the formation of DNA adducts. N7-(2-carbamoyl-2-hydroxyethyl) guanine (N7-GAG), the most abundant DNA adduct induced by GA, was recently detected in urine of smokers and non-smokers. In this study, we assessed the variability of AA exposure and biomarkers of AA exposure in urine samples repeatedly collected from AA-exposed workers and explored the half-life of N7-GAG. A total of 8 AA-exposed workers and 36 non-exposed workers were recruited. Pre-shift and post-shift urine samples were collected from the exposed group in parallel with personal sampling for eight consecutive days and from the control group on day 1 of the study. Urinary N7-GAG and the mercapturic acids of AA and GA, namely N-acetyl-S-(2-carbamoylethyl)-L-cysteine (AAMA) and N-(R,S)-acetyl-S-(1-carbamoyl-2-hydroxyethyl)-L-cysteine (GAMA) were analyzed using on-line solid phase extraction-liquid chromatography-electrospray ionization/tandem mass spectrometry methods. We found that N7-GAG levels in urine were significantly higher in exposed wo...Continue Reading

References

Jan 1, 1986·Annual Review of Genetics·L A Loeb, B D Preston
Aug 1, 1995·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·M A FriedmanM A Stedham
Aug 1, 1995·The Annals of Occupational Hygiene·S M RappaportL L Kupper
Nov 24, 1999·Chemical Research in Toxicology·S C SumnerB I Ghanayem
Mar 21, 2000·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·C J SmithD J Doolittle
Aug 9, 2002·Journal of Agricultural and Food Chemistry·Eden TarekeMargareta Törnqvist
Oct 21, 2003·Chemical Research in Toxicology·Gonçalo Gamboa da CostaDaniel R Doerge
Apr 20, 2004·Toxicology Letters·Peter B Farmer
Jul 9, 2004·Journal of the National Cancer Institute·Ahmad Besaratinia, Gerd P Pfeifer
Dec 31, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Timothy R FennellMarvin A Friedman
Jan 26, 2005·Mutation Research·Isabelle ManièreJean-Michel Poul
Jan 26, 2005·Mutation Research·Daniel R DoergeFrederick A Beland
Jun 16, 2005·Environmental and Molecular Mutagenesis·Mugimane G ManjanathaDaniel R Doerge
Sep 6, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Burhan I GhanayemDaniel R Doerge
Oct 20, 2005·Occupational and Environmental Medicine·Y S LinS M Rappaport
Feb 24, 2006·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Uwe FuhrEdgar Schömig
Jan 30, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Thomas BjellaasGeorg Becher
Nov 17, 2007·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Janneke G HogervorstPiet A van den Brandt
Apr 18, 2008·Archives of Toxicology·Eva SettelsKlaus E Appel
Jul 16, 2008·Journal of Agricultural and Food Chemistry·Eva C HartmannJürgen Angerer
May 26, 2009·Mutation Research·Gunnar BoysenJames A Swenberg
Feb 23, 2010·Critical Reviews in Toxicology·Janneke G F HogervorstPiet A van den Brandt
Jul 3, 2010·International Archives of Occupational and Environmental Health·Yu-Fang HuangMei-Lien Chen
Feb 18, 2011·Mass Spectrometry Reviews·Kuen-Yuh WuJames A Swenberg
Mar 29, 2011·Mutagenesis·Naoki KoyamaMasamitsu Honma
May 23, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Belinda J NixonShaun D Roman

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Methods Mentioned

BETA
nuclear magnetic resonance
urine collection

Software Mentioned

SPSS

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