PMID: 12788650Jun 6, 2003Paper

Feasibility, sensitivity, and reliability of laser-induced fluorescence imaging of green fluorescent protein-expressing tumors in vivo

Molecular Therapy : the Journal of the American Society of Gene Therapy
Severine WackMarc Aprahamian

Abstract

Whole-body imaging of green fluorescent protein (GFP) can be used to test the efficiency of gene carriers for in vivo transduction. The aim of the current study was to determine the sensitivity and the accuracy of a GFP imaging procedure by in vivo investigation of GFP-expressing tumor cells. An improved method of whole-body GFP imaging made use of a laser excitation source and band-pass filters matched specifically to GFP and constitutive tissue fluorescence emission bands. Processing of the primary GFP fluorescence images acquired by the CCD camera subtracted background tissue autofluorescence. Our approach achieved 100% sensitivity and specificity for in vivo detection of 10%-transfected BxPc3 pancreatic tumor after subcutaneous grafting or orthotopical implantation in the pancreas of nude mice. It also detected less transfected tumors (i.e., 1 to 5%) but with a loss in sensitivity (50% of cases). The system was employed over a 5-week period to monitor the persistence of GFP expression in 10%-transfected BxPc3 tumors orthotopically implanted in the pancreas of two nude mice, allowing the direct visualization of tumor progression and spread. In facilitating the temporal-spatial follow-up of GFP expression in vivo, the optimiz...Continue Reading

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Citations

Oct 1, 2009·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Sevim KahramanSalih Sanlioglu
Oct 1, 2005·Nature Reviews. Cancer·Robert M Hoffman
Aug 19, 2004·Photomedicine and Laser Surgery
Oct 19, 2011·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Elizabeth M C HillmanRichard M Levenson
Jun 23, 2006·Neoplasia : an International Journal for Oncology Research·Sharon Moshitch-MoshkovitzIlan Tsarfaty
Dec 25, 2009·Journal of Photochemistry and Photobiology. B, Biology·Frederic LeblondBrian W Pogue
May 18, 2011·Diabetes/metabolism Research and Reviews·Sevim KahramanAhter D Sanlioglu
Jun 21, 2015·Fungal Genetics and Biology : FG & B·M SchusterG Steinberg
Nov 7, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Séverine WackAmor Hajri
Jan 16, 2016·Physical Chemistry Chemical Physics : PCCP·Jae Woo Park, Young Min Rhee
Sep 24, 2005·Journal of Biomedical Optics·Robert M Hoffman

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