Features of cell degeneration and death in hepatic failure and systemic lymphoid depletion characteristic of porcine circovirus-2-associated postweaning multisystemic wasting disease

Veterinary Pathology
S KrakowkaG M Allan

Abstract

Tissue section replicates from lymphoid tissues and livers of gnotobiotic swine were examined by immunohistochemistry for the colocalization of porcine circovirus-2 (PCV-2) nucleocapsid and terminal deoxynucleotidyl transferase (TdT)-mediated incorporation of biotinylated nucleotides (UTP) onto the 3'-exposed hydroxyl groups (nick end labeling) nuclear deoxyribonucleic acid (TUNEL), a marker for apoptosis. Single- and dually stained replicates from uninfected controls, subclinically affected PCV-2-infected gnotobiotic pigs, PCV-2-infected piglets immunosuppressed with cyclosporine (Cys), and PCV-2-infected piglets with post-weaning multisystemic wasting syndrome (PMWS) were evaluated. Thymuses were used as positive controls for apoptosis absent PCV-2, tissue sections from dogs given hyperthermic stress were examined as positive controls for induced TUNEL. Tissues from heat-stressed dogs contained TUNEL-positive cell nuclei in both lymphoid tissues and liver, TUNEL was greatest shortly after the delivery of the hyperthermic insult. In uninfected control and subclinically affected PCV-2-infected gnotobiotic pigs, rare hepatocytes and lymphoid cells were TUNEL positive, the frequency of these was similar to that seen in uninfected...Continue Reading

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Citations

Feb 25, 2010·The Journal of Clinical Investigation·Karine LabadiePierre Roques
Sep 3, 2010·Journal of Virology·Mark ChaiyakulMarkus Czub
Dec 20, 2011·Virus Research·Laila Darwich, Enric Mateu
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Feb 27, 2014·Veterinary Pathology·J Ellis
Jun 14, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Steven KrakowkaGordon Allan
Apr 4, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Alexander HambergSteven Krakowka
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Sep 3, 2008·Animal Health Research Reviews·Sheela Ramamoorthy, Xiang-Jin Meng
Sep 21, 2018·Frontiers in Microbiology·Yuhong PanAnchun Cheng

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