Features of the milk whey protein partitioning in polyethyleneglycol-sodium citrate aqueous two-phase systems with the goal of isolating human alpha-1 antitrypsin expressed in bovine milk

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Andrea BoaglioB Nerli

Abstract

Partitioning behaviour of the bovine whey proteins (bovine serum albumin, alpha-lactoalbumin and beta-lactoglobulin) and human alpha-1 antitrypsin in aqueous two-phase systems prepared with polyethyleneglycol (molecular masses: 1000, 1450 and 3350)-sodium citrate was analysed at pH 5.2, 6.2 and 8.2. Alpha lactoalbumin concentrated in the polyethyleneglycol rich-phase, while beta-lactoglobulin, bovine serum albumin and alpha-1 antitrypsin showed affinity for the citrate rich-phase. In aqueous two-phase systems of high medium pH and high polyethyleneglycol molecular mass the protein partitioning equilibrium is displaced to the citrate rich-phase. The polyethyleneglycol 1450-pH 5.2 system with a top/bottom phase-volume ratio of 3 showed to have the best capability of recovering the alpha-1 antitrypsin from a mixture prepared with natural milk whey and human alpha-1 antitrypsin. The recovery of this protein in the bottom phase was of 90% and the purity of the obtained product was of 98%. The method appears to be suitable as a starting point to isolate other human proteins expressed in transgenic bovine milk.

References

Aug 12, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·M Rito-Palomares, M Hernandez
Oct 29, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Georgina RehGuillermo Picó

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Citations

May 2, 2013·Chemistry Central Journal·Sunantha KetnawaSaroat Rawdkuen
May 31, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lizzy Ayra Pereira AlcântaraMaria do Carmo Hespanhol da Silva
Mar 1, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Babak MokhtaraniMohammad Hassan Amini
Oct 5, 2010·Bioresource Technology·Jonnalagadda Raghava Rao, Balachandran Unni Nair
Dec 26, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gisela TubioBibiana B Nerli
May 20, 2021·Biomacromolecules·Shunsuke OnogiKenneth J Shea

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