PMID: 9546796Apr 18, 1998Paper

Features of the SIVmac transmembrane glycoprotein cytoplasmic domain that are important for Env functions

AIDS Research and Human Retroviruses
B L ShacklettP Sonigo

Abstract

The cytoplasmic domain (CD) of the SIVmac transmembrane protein (TM) can affect viral infectivity by modulating several Env functions, notably fusogenic capacity and incorporation into virions. In addition, envelopes with a truncated CD are counterselected in primary cells in culture and in vivo in rhesus macaques, suggesting a role for this domain in viral persistence. Here, we have used mutagenesis to examine specific features of the SIVmac TM CD, including the conserved C-terminal alpha helix and the overall length of the CD. Several mutations dramatically reduced and/or delayed virus infectivity in lymphoid cell lines. Detailed analysis of mutants revealed defects in envelope stability, fusogenic capacity, and virion incorporation. The primary defect associated with an envelope containing a 64-residue CD was rapid degradation. A mutant Env lacking the C-terminal alpha helix but encoding an exceptionally long CD (373 residues) was highly fusogenic but inefficiently incorporated into virions. A third mutant, containing amino acid substitutions designed to alter the charge density of the C-terminal helix, retained cytopathic properties and showed enhanced fusogenic capacity but replicated with delayed kinetics. Taken together,...Continue Reading

References

May 1, 1990·Journal of Virology·D H SpectorS A Spector
Nov 1, 1990·AIDS Research and Human Retroviruses·D A Regier, R C Desrosiers
Oct 19, 1989·Nature·V M HirschJ I Mullins
Aug 1, 1989·AIDS Research and Human Retroviruses·W R GallaherR C Montelaro
Nov 1, 1989·Journal of Virology·T KodamaR C Desrosiers
Mar 1, 1993·AIDS Research and Human Retroviruses·J B Jowett, I M Jones
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·C P HillW I Sundquist

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Citations

Aug 10, 2000·AIDS Research and Human Retroviruses·R D BerkowitzJ M McCune
Oct 29, 2000·AIDS Research and Human Retroviruses·J MillsN Almond

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