Fecal PCR assay for diagnosis of Helicobacter infection in laboratory rodents.

Journal of Clinical Microbiology
C S BeckwithL K Riley

Abstract

A fecal PCR assay for detection of Helicobacter infections in laboratory rodents was developed. DNA was isolated from murine fecal pellets, and a region of the 16S rRNA gene conserved among murine Helicobacter species was amplified. The fecal PCR was sensitive and specific. This assay does not require euthanasia of rodents, which is especially important for valuable rodents, such as transgenic mice.

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Citations

Nov 28, 2002·Experimental Animals·Toshinori FurukawaEiko Okada
May 3, 2002·Clinical and Diagnostic Laboratory Immunology·Sunlian FengStephen W Barthold
Aug 31, 2001·ILAR Journal·Steven H. WeisbrothWilliam Shek
May 29, 2008·ILAR Journal·David G BesselsenLela K Riley
Dec 18, 2002·Helicobacter·Ulrich R M BohrPeter Malfertheiner
Jul 4, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dilini C RanatungaJay H Bream
Jan 19, 2000·The Journal of Experimental Medicine·M J NicklinG W Duff
Aug 28, 2020·Anais Da Academia Brasileira De Ciências·Gabriel E Matos-RodriguesRodrigo A P Martins
Jun 11, 1999·The American Journal of Physiology·M SchultzR B Sartor
Jan 25, 2018·Scandinavian Journal of Medicine & Science in Sports·Xin YuanShaozhen Hou
Mar 31, 1999·Comparative Immunology, Microbiology and Infectious Diseases·L Zenner
Oct 30, 2001·Journal of Clinical Microbiology·C L FranklinJ G Fox

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