Fecal transplant modifies urine chemistry risk factors for urinary stone disease

Physiological Reports
Joshua M SternSylvia O Suadicani

Abstract

Urinary stone disease (USD) is a major health concern. There is a need for new treatment modalities. Recently, our group provided evidence for an association between the GMB composition and USD. The accessibility of the Gut Microbiome (GMB) makes it an attractive target for investigation and therefore, in these studies we have evaluated the extent to which the whole gut microbial community in fecal transplants can affect urinary stone risk parameters in an animal model. Fresh fecal pellets were collected from Zucker lean rats, homogenized in PBS (100 mg/mL), filtered through a 70 μm strainer and then orally gavaged into C57BL/6NTac germ-free mice. Twenty-four hours urine collections and GMB analysis were performed over time for 1 month. Kidney and gut tissue were harvested from transplanted mice for western blot analysis of expression levels of the Slc26a6 transporter involved in oxalate balance. Urinary calcium decreased after fecal transplant by 55% (P < 0.001). Urinary oxalate levels were on average 24% lower than baseline levels (P < 0.001). Clostridiaceae family was negatively correlated with urinary oxalate at 4 weeks after transplant (r = -0.83, P < 0.01). There was a 0.6 unit average increase in urinary pH from a baseli...Continue Reading

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Citations

May 31, 2019·Current Opinion in Nephrology and Hypertension·Menghan Liu, Lama Nazzal
May 31, 2019·Current Opinion in Nephrology and Hypertension·Shivam Joshi, David S Goldfarb
Feb 6, 2021·Mucosal Immunology·Bernadette Jones-FreemanMalcolm R Starkey
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Jul 9, 2021·Kidney Research and Clinical Practice·Myung-Gyu KimSang-Kyung Jo

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Methods Mentioned

BETA
PCR
urine collection

Software Mentioned

ggplot2
uclust
vegan
BLAST
R
VSEARCH
PyNAST
prinseq
FastTree
PERMANOVA

Related Concepts

Related Feeds

Bacteriotherapy (ASM)

Bacteriotherapy, also known as fecal transplantation, involves the transfer of stool from a healthy donor into the gastrointestinal tract for the purpose of treating recurrent C. difficile colitis. Here is the latest on bacteriotherapy.

Bacteriotherapy

Bacteriotherapy, also known as fecal transplantation, involves the transfer of stool from a healthy donor into the gastrointestinal tract for the purpose of treating recurrent C. difficile colitis. Here is the latest on bacteriotherapy.

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