FecX Bar a Novel BMP15 mutation responsible for prolificacy and female sterility in Tunisian Barbarine Sheep

BMC Genetics
Narjess LassouedSonia Bedhiaf-Romdhani

Abstract

Naturally occurring mutations in growth and differentiation factor 9 (GDF9) or bone morphogenetic protein 15 (BMP15) genes are associated with increased ovulation rate (OR) and litter size (LS) but also sterility. Observing the Tunisian Barbarine ewes of the "W" flock selected for improved prolificacy, we found prolific and infertile ewes with streaky ovaries. Blood genomic DNA was extracted from a subset of low-ovulating, prolific and infertile ewes of the "W" flock, and the entire coding sequences of GDF9 and BMP15 were sequenced. We evidenced a novel polymorphism in the exon 1 of the BMP15 gene associated with increased prolificacy and sterility. This novel mutation called FecX Bar is a composite polymorphism associating a single nucleotide substitution (c.301G > T), a 3 bp deletion (c.302_304delCTA) and a C insertion (c.310insC) in the ovine BMP15 cDNA leading to a frame shift at protein position 101. Calculated in the "W" flock, the FecX Bar allele increased OR by 0.7 ova and LS by 0.3 lambs (p = 0.08). As for already identified mutations, homozygous females carrying FecX Bar exhibited streaky ovaries with a blockade at the primary stage of folliculogenesis as shown by histochemistry. Our investigation demonstrates a new m...Continue Reading

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Citations

Nov 24, 2019·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Asrat Tera DoleboJoram M Mwacharo
Nov 7, 2019·Animals : an Open Access Journal From MDPI·Hang YinQifa Li
Feb 27, 2020·Animal : an International Journal of Animal Bioscience·A HaileB Rischkowsky
Apr 1, 2019·Veterinary World·Sara E Ibrahim MohamedMohammed-Khair A Ahmed
Mar 8, 2020·Animals : an Open Access Journal From MDPI·Wilber Hernández-MontielRoberto Zamora-Bustillos
Jul 9, 2020·Frontiers in Genetics·Louise ChantepieStéphane Fabre
May 21, 2019·Frontiers in Genetics·Karen MarshallStephen J Kemp
Feb 11, 2021·Reproduction in Domestic Animals = Zuchthygiene·Juliana de Carvalho DelgadoMayra Elena Ortiz D'Ávila Assumpção
Mar 7, 2021·Animals : an Open Access Journal From MDPI·Fengyan WangRan Di

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Methods Mentioned

BETA
genotyping
PCR
light microscopy

Software Mentioned

CLC MainWorkbench

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