Feed Additives Differentially Impact the Epimural Microbiota and Host Epithelial Gene Expression of the Bovine Rumen Fed Diets Rich in Concentrates

Frontiers in Microbiology
R M PetriQ Zebeli

Abstract

The success of nutritional strategies for the prevention of subacute ruminal acidosis (SARA) and the related microbial dysbiosis still remains unpredictable due to the complexity of the rumen ecosystem. The rumen epimural community, due to proximity, has the greatest opportunity to influence host gene expression. The aim of this study was to determine the effect of two separate feed additives on the rumen epimural community and host epithelial gene expression. Eight rumen cannulated Holstein cows were randomly assigned to one of three feeding groups: autolyzed yeast (AY), phytogenics (PHY) and control (CON) using a 3 × 3 Latin square design. Cows were fed an intermittent SARA model that started with 100% forage diet (Baseline) followed by two 65% concentrate-diet induced SARA challenges (SARAI, SARAII), separated by 1 week of forage only feeding. Rumen papillae samples were collected via the cannula during the Baseline, SARAI and SARAII periods. Microbial DNA was extracted and sequenced targeting the 16S rRNA gene and host RNA was analyzed using RT-qPCR. Analysis of the taxonomic composition at the genera level showed a tendency to increase in the relative abundances of Pseudobutyrivibrio (P = 0.06), Selenomonas (P = 0.07) and ...Continue Reading

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Citations

Feb 13, 2021·Microorganisms·Cátia PacíficoQendrim Zebeli
Feb 6, 2021·Animals : an Open Access Journal From MDPI·Nicole C Burdick SanchezJeffery A Carroll

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Datasets Mentioned

BETA
PRJEB33839
PRJEB29866

Methods Mentioned

BETA
biopsies
Assay
PCR
RNA Assay

Software Mentioned

PrimerBLAST
BestKeeper
QIIME
LEfSe
NormFinder
SAS
R
Vegan

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