PMID: 2495790Mar 1, 1989Paper

Feedback control of ornithine decarboxylase expression by polyamines. Analysis of ornithine decarboxylase mRNA distribution in polysome profiles and of translation of this mRNA in vitro

The Biochemical Journal
I HolmO Heby

Abstract

Cell growth and differentiation require the presence of optimal concentrations of polyamines. Ornithine decarboxylase (ODC) catalyses the first and rate-controlling step in polyamine synthesis. In studies using cultures of Ehrlich ascites-tumour cells, we have shown that the expression of ODC is subject to feedback regulation by the polyamines. A decrease in the cellular polyamine concentration results in a compensatory increase in the synthesis of ODC, whereas an increase in polyamine concentration results in suppression of ODC synthesis. These changes in ODC synthesis were attributed to changes in the efficiency of ODC mRNA translation, because the steady-state amount of ODC mRNA remained constant. We now show that the number of ribosomes associated with ODC mRNA is low, and that the increase in ODC mRNA translation takes place without a shift in the distribution of ODC mRNA towards larger polysomes. This finding indicates that the polyamines regulate the efficiency of ODC mRNA translation by co-ordinately affecting the rates of initiation and elongation. By analysing ODC mRNA translation in vitro, using a rabbit reticulocyte lysate, polyadenylated RNA from a cell line with an amplified ODC gene, and a monospecific anti-ODC a...Continue Reading

Citations

Nov 1, 1994·Agents and Actions·A Sessa, A Perin
Oct 23, 1989·FEBS Letters·K L Manchester
Dec 21, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·Q YuanL R Johnson
May 2, 2001·The International Journal of Biochemistry & Cell Biology·P T KilpeläinenO A Hietala
Apr 27, 1999·The International Journal of Biochemistry & Cell Biology·L M Shantz, A E Pegg
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Apr 1, 1990·Trends in Biochemical Sciences·O Heby, L Persson
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Aug 15, 1991·Biochemical and Biophysical Research Communications·K KashiwagiK Igarashi
Oct 1, 1991·Archives of Biochemistry and Biophysics·J L MitchellA Bareyal-Leyser
Apr 30, 1992·Biochemical and Biophysical Research Communications·A SuorsaA Pajunen

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