Feedback inhibition of nitrogenase.

Journal of Bacteriology
J K GordonW J Brill

Abstract

No inhibition of nitrogenase activity by physiological levels of NH4+ or carbamyl phosphate was observed in extracts of Azotobacter vinelandii. All of the 15N2 reduced by cultures which received no NH4+ was found in the cells. By contrast, more than 95% of the 15N2 reduced by cultures which had been given NH4+ was found in the medium. Failure to examine the culture medium would lead to the erroneous conclusion that N2 fixation is inhibited by NH4+. Nitrogenase in a derepressed mutant strain of A. vinelandii was fully active in vivo in the presence of NH4+. The addition of NH4Cl to N2-fixing cultures resulted in no decrease in the N2-reducing activity of intact cells of Klebsiella pneumoniae or Clostridium pasteurianum and only a small (15%) decrease in A. vinelandii. Therefore, no significant inhibition of nitrogenase by NH4+ or metabolites derived from NH4+ exists in A. vinelandii, K. pneumoniae, or C. pasteurianum.

References

Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P W Ludden, R H Burris
Apr 27, 1978·Archives of Microbiology·W G Zumft, F Castillo
Jul 17, 1973·Biochemical and Biophysical Research Communications·B Seto, L E Mortenson
Feb 1, 1974·Journal of Bacteriology·B L Seto, L E Mortenson
Jan 1, 1968·Canadian Journal of Microbiology·G W Strandberg, P W Wilson
Sep 1, 1966·Journal of Bacteriology·D C Yoch, R M Pengra
Feb 1, 1981·Journal of Bacteriology·W J Sweet, R H Burris
Jan 1, 1981·Annual Review of Microbiology·G P Roberts, W J Brill
Dec 1, 1959·Canadian Journal of Microbiology·D W WESTLAKE, P W WILSON
Mar 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·W A BULENJ R LECOMTE
Nov 18, 1960·Biochimica Et Biophysica Acta·J E CARNAHANJ E CASTLE
Nov 1, 1946·Journal of Bacteriology·R H Burris, P W Wilson
Sep 1, 1951·Proceedings of the National Academy of Sciences of the United States of America·I Zelitch
Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J K Gordon, W J Brill

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Citations

Jun 1, 1989·Journal of Bacteriology·H Fu, R H Burris
Jan 1, 1984·Journal of Bacteriology·J Klugkist, H Haaker
Mar 1, 1990·Applied and Environmental Microbiology·R S Oremland
Mar 1, 1986·Journal of Bacteriology·A HartmannR H Burris

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