Feedback regulation of SIN by Etd1 and Rho1 in fission yeast

Genetics
María Alcaide-GavilánJuan Jimenez

Abstract

In fission yeast, the septation initiation network (SIN) is thought to promote cytokinesis by downstream activation of Rho1, a conserved GTPase that controls cell growth and division. Here we show that Etd1 and PP2A-Pab1, antagonistic regulators of SIN, are Rho1 regulators. Our genetic and biochemical studies indicate that a C-terminal region of Etd1 may activate Rho1 by directly binding it, whereas an N-terminal domain confers its ability to localize at the growing tips and the division site where Rho1 functions. In opposition to Etd1, our results indicate that PP2A-Pab1 inhibits Rho1. The SIN cascade is upstream-regulated by the Spg1 GTPase. In the absence of Etd1, activity of Spg1 drops down prematurely, thereby inactivating SIN. Interestingly, we find that ectopic activation of Rho1 restores Spg1 activity in Etd1-depleted cells. By using a cytokinesis block strategy, we show that Rho1 is essential to feedback-activate Spg1 during actomyosin ring constriction. Therefore, activation of Spg1 by Rho1, which in turn is regulated by Etd1, uncovers a novel feedback loop mechanism that ensures SIN activity while cytokinesis is progressing.

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Citations

Mar 5, 2016·World Journal of Microbiology & Biotechnology·Rebeca Martín-García, Beatriz Santos
Oct 21, 2015·Seminars in Cell & Developmental Biology·Vasudha SrivastavaDouglas N Robinson
May 8, 2016·Cellular Microbiology·Pilar PérezJuan C Ribas
Aug 4, 2018·Journal of Cell Science·Sumit K Dey, Thomas D Pollard
Nov 2, 2019·Communicative & Integrative Biology·Brian Hercyk, Maitreyi Das
Jun 11, 2020·Molecular Biology of the Cell·Samantha E R Dundon, Thomas D Pollard
Jan 8, 2020·International Journal of Molecular Sciences·Yolanda Moyano-Rodriguez, Ethel Queralt
Dec 17, 2014·Journal of Cell Science·Paulina WachowiczViesturs Simanis
Jul 3, 2021·Cells·Jero Vicente-SolerMarisa Madrid

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