Feeder-free adaptation, culture and passaging of human IPS cells using complete Knockout Serum Replacement feeder-free medium.

Journal of Visualized Experiments : JoVE
Kate Wagner, David Welch

Abstract

The discovery in 2006 that human and mouse fibroblasts could be reprogrammed to generate iPS cells with qualities remarkably similar to embryonic stem cells has created a valuable new source of pluripotent cells for drug discovery, cell therapy, and basic research. GIBCO media and reagents have been at the forefront of pluripotent stem cell research for years. Knockout DMEM supplemented with Knockout Serum Replacement is the media of choice for embryonic stem cell growth and now iPS cell culture. This gold standard media system can now be used for feeder-free culture with the addition of Knockout SR Growth Factor Cocktail. Traditional human ES and iPS cell culture methods require the use of mouse or human fibroblast feeder layers, or feeder-conditioned medium. These culture methods are labor-intensive, hard to scale and it is difficult to maintain hiPS cells undifferentiated due to the undefined conditions. Invitrogen has developed Knockout SR Growth Factor Cocktail to allow you to easily transition your hiPS cell cultures to feeder-free while still maintaining your use of Knockout SR.

References

Nov 22, 2007·Science·Junying YuJames A Thomson
Jul 5, 2008·Nature Protocols·In-Hyun ParkGeorge Q Daley
Oct 22, 2008·Nature Biotechnology·Trond AasenJuan Carlos Izpisúa Belmonte
Dec 2, 2008·Cell Stem Cell·Nimet Maherali, Konrad Hochedlinger
Dec 23, 2008·Cell Stem Cell·Jing LiaoLei Xiao

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Citations

Aug 13, 2013·DNA and Cell Biology·Jingjing GuoXingchang Hu
Jun 24, 2010·Cell Stem Cell·Kouichi HasegawaMartin F Pera
Jan 18, 2018·Scientific Reports·Jack W LambsheadAndrew L Laslett
Jan 18, 2020·Molecular Biology Reports·Deepti SinghMichael Young

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