The differentiation of self-renewing progenitor cells requires not only the regulation of lineage- and developmental stage-specific genes but also the coordinated adaptation of housekeeping functions from a metabolically active, proliferative state toward quiescence. How metabolic and cell-cycle states are coordinated with the regulation of cell type-specific genes is an important question, because dissociation between differentiation, cell cycle, and metabolic states is a hallmark of cancer. Here, we use a model system to systematically identify key transcriptional regulators of Ikaros-dependent B cell-progenitor differentiation. We find that the coordinated regulation of housekeeping functions and tissue-specific gene expression requires a feedforward circuit whereby Ikaros down-regulates the expression of Myc. Our findings show how coordination between differentiation and housekeeping states can be achieved by interconnected regulators. Similar principles likely coordinate differentiation and housekeeping functions during progenitor cell differentiation in other cell lineages.
A conserved degradation signal regulates RAG-2 accumulation during cell division and links V(D)J recombination to the cell cycle
Identification of genes associated with chemotherapy crossresistance and treatment response in childhood acute lymphoblastic leukemia
Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles
maSigPro: a method to identify significantly differential expression profiles in time-course microarray experiments
Acquisition and extinction of gene expression programs are separable events in heterokaryon reprogramming
Ikaros DNA-binding proteins as integral components of B cell developmental-stage-specific regulatory circuits
Integration of genomic and gene expression data of childhood ALL without known aberrations identifies subgroups with specific genetic hallmarks
A subtype of childhood acute lymphoblastic leukaemia with poor treatment outcome: a genome-wide classification study
A global network of transcription factors, involving E2A, EBF1 and Foxo1, that orchestrates B cell fate
HIF1alpha-dependent glycolytic pathway orchestrates a metabolic checkpoint for the differentiation of TH17 and Treg cells
The sva package for removing batch effects and other unwanted variation in high-throughput experiments
A self-reinforcing regulatory network triggered by limiting IL-7 activates pre-BCR signaling and differentiation
Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks
The B-cell identity factor Pax5 regulates distinct transcriptional programmes in early and late B lymphopoiesis
Transcript dynamics of proinflammatory genes revealed by sequence analysis of subcellular RNA fractions
IKAROS deletions dictate a unique gene expression signature in patients with adult B-cell acute lymphoblastic leukemia
Genome-wide identification of Ikaros targets elucidates its contribution to mouse B-cell lineage specification and pre-B-cell differentiation.
TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions
Independent prognostic value of BCR-ABL1-like signature and IKZF1 deletion, but not high CRLF2 expression, in children with B-cell precursor ALL.
Wellington: a novel method for the accurate identification of digital genomic footprints from DNase-seq data
Orchestrating B cell lymphopoiesis through interplay of IL-7 receptor and pre-B cell receptor signalling
Loss of Ikaros DNA-binding function confers integrin-dependent survival on pre-B cells and progression to acute lymphoblastic leukemia
The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells
Sox5 and c-Maf cooperatively induce Th17 cell differentiation via RORγt induction as downstream targets of Stat3
dslice: an R package for nonparametric testing of associations with application in QTL and gene set analysis
Coordinating cell proliferation and differentiation: Antagonism between cell cycle regulators and cell type-specific gene expression
Cycling through developmental decisions: how cell cycle dynamics control pluripotency, differentiation and reprogramming
Tissue-Specificity of Gene Expression Diverges Slowly between Orthologs, and Rapidly between Paralogs
Cell circuits between B cell progenitors and IL-7+ mesenchymal progenitor cells control B cell development
EBF1 and Pax5 safeguard leukemic transformation by limiting IL-7 signaling, Myc expression, and folate metabolism
B cells: Gene Expression
B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.
B-Cell Leukemia (Keystone)
B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.
Adult Stem Cells
Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.