Feeding of Enterococcus faecium NCIMB 10415 Leads to Intestinal miRNA-423-5p-Induced Regulation of Immune-Relevant Genes

Applied and Environmental Microbiology
Susanne Kreuzer-RedmerGudrun A Brockmann

Abstract

Probiotics are widely used in human and animal health, but little is known about the mode of action of probiotics. One possible mechanism at the molecular level could be an influence on microRNAs (miRNAs) and the related immune-relevant target genes. Here, we analyzed differential expression of miRNA and potential target genes of ileal and jejunal lymphatic tissues from Enterococcus faeciumNCIMB 10415-fed piglets versus untreated controls by using next-generation sequencing. We identified miR-423-5p as being greatly affected by the treatment group (2.32-fold;P= 0.014). Validation by reverse transcription-quantitative PCR (RT-qPCR) confirmed a significant upregulation of miR-423-5p (2.11-fold;P= 0.03) and, additionally, downregulation of the important immune-relevant immunoglobulin lambda light C region (IGLC) (0.61-fold;P= 0.03) and immunoglobulin kappa constant (IGKC) (0.69-fold;P= 0.04) target genes. Expression analysis of miR-423-5p and IGLC at different age points shows a clear anti correlated relationship. Luciferase reporter assays with a HeLa cell line verified IGLC as a target of miR-423-5p. The results provided evidence for an effect of feeding of E. faeciumon the expression of miR-423-5p and on the regulation of the I...Continue Reading

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Citations

Nov 25, 2016·Molecular Nutrition & Food Research·Amel TaibiElena M Comelli
Oct 27, 2017·Nutrients·Amy Llewellyn, Andrew Foey
Apr 3, 2019·International Journal of Molecular Sciences·Jingyu WangChunlai Jiang
Jan 13, 2021·Cell Communication and Signaling : CCS·Amirhossein DavoodvandiHamed Mirzaei
Apr 15, 2020·Microbial Pathogenesis·Ava BehrouziSeyed Davar Siadat

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