Feline embryo development in commercially available human media supplemented with fetal bovine serum

The Journal of Veterinary Medical Science
Md Emtiaj AlamShingo Hatoya

Abstract

Feline embryo development was examined for 7 days after fertilization using commercially available human media supplemented with 0.3% bovine serum albumin (BSA) or 5% fetal bovine serum (FBS). Cumulus-oocyte complexes were categorized as Grades 1, 2, and 3 according to morphology. Only-One Medium (OM) was used for in vitro culture (IVC) in OM + BSA, OM + FBS, and OM + BSA/FBS, with BSA supplementation for the first 2 days and FBS for the subsequent 5 days. Embryos cultured in Early Culture Medium (1-2 days) and Blastocyst Medium (3-7 days) were defined as EB + BSA and EB + BSA/FBS. The developmental rate until the blastocyst stage of Grade 1 and 2 oocytes cultured in OM + BSA/FBS was higher than for the other groups and was significantly higher than for the OM + BSA and EB + BSA groups (P<0.01). Grade 3 oocytes cultured in OM + BSA/FBS also showed the greatest proportion of blastocyst formation. However, FBS supplementation throughout the IVC period reduced blastocyst number. The percentage of 2 pronuclei after fertilization as well as blastocyst cell number were significantly higher in Grade 1 and 2 than Grade 3 oocytes when cultured in OM + BSA/FBS (P<0.05). These results indicate that commercially available OM supplemented w...Continue Reading

References

Jan 1, 1990·Molecular Reproduction and Development·B D Bavister, T Arlotto
Nov 7, 2002·Reproductive Biomedicine Online·John D Biggers, Catherine Racowsky
Dec 7, 2002·Reproduction, Fertility, and Development·N W Kurniani KarjaTatsuyuki Suzuki
Nov 19, 2005·The Journal of Reproduction and Development·Tatsuo FujitaKazuho Shiga
Apr 20, 2006·Theriogenology·C E PopeB L Dresser
Dec 2, 2006·The Journal of Reproduction and Development·Hideaki NaoiTakashi Nagai
Jul 9, 2010·Reproductive Biology and Endocrinology : RB&E·Goedele PaternotCarl Spiessens
Dec 22, 2010·The Journal of Veterinary Medical Science·Kenzo UchikuraMitsugu Hishinuma

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