Feline immunodeficiency virus Orf-A localizes to the nucleus and induces cell cycle arrest

Virology
Malou C GemenianoEllen E Sparger

Abstract

Feline immunodeficiency virus (FIV) gene orf-A, also designated orf-2, encodes a 77 amino acid accessory protein reported to be critical for efficient viral replication in vitro and in vivo and previously implicated to encode a Tat protein for FIV. However, recent studies have shown Orf-A to be important in the late steps of the FIV life cycle involved in virion formation and in early steps involved in virus infectivity. The present study reports that expression of a GFP-Orf-A fusion protein in both primate and feline cell lines results in nuclear localization of this FIV accessory protein. Moreover, a nuclear localization signal (NLS) critical for nuclear import was mapped to amino acid residues 43 through 53 of Orf-A. Lastly, transient expression of GFP-Orf-A in cells induced an arrest at the second gap (G(2)) of the cell cycle. Our findings reveal that Orf-A is a nuclear protein that expresses properties similar to those reported for human immunodeficiency virus-1 (HIV-1)-encoded Vpr.

References

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Jul 30, 2003·Journal of Virology·Malou C GemenianoEllen E Sparger

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Citations

Oct 17, 2006·Clinical Microbiology Reviews·Sue VandeWoude, Cristian Apetrei
Jul 16, 2008·Nucleic Acids Research·Laurie James, Bruno Sargueil
Mar 24, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ferdinand MaingatChristopher Power
Nov 10, 2011·Viruses·Jörg Zielonka, Carsten Münk
Apr 7, 2007·American Journal of Respiratory Cell and Molecular Biology·Xiaoling YuanYuwen Cong
Jul 9, 2013·Retrovirology·Samantha J McDonnelBrian G Murphy
Jul 12, 2011·Veterinary Immunology and Immunopathology·Reinhard ErtlDieter Klein
Jul 28, 2009·Biochimica Et Biophysica Acta·Laurent BalvayThéophile Ohlmann
Feb 22, 2008·Veterinary Immunology and Immunopathology·John H ElderYing-Chuan Lin
Feb 26, 2008·Veterinary Immunology and Immunopathology·Elizabeth W UhlJanet K Yamamoto
Aug 21, 2007·Comparative Immunology, Microbiology and Infectious Diseases·Sylvie Lecollinet, Jennifer Richardson
May 9, 2006·Veterinary Immunology and Immunopathology·Stephen P Dunham
Aug 8, 2015·Viruses·Bárbara Rojas-ArayaRicardo Soto-Rifo
Apr 21, 2018·Viruses·Craig MillerSue VandeWoude
May 10, 2013·Journal of Virology·Ryan M TroyerSue VandeWoude
Feb 23, 2012·Viruses·Hind J FadelEric M Poeschla

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