PMID: 7545246Oct 1, 1995Paper

Feline immunodeficiency virus reverse transcriptase: expression, functional characterization, and reconstitution of the 66- and 51-kilodalton subunits

Journal of Virology
M AmackerU Hübscher

Abstract

The two subunits of the feline immunodeficiency virus (FIV) reverse transcriptase (RT) were cloned and functionally expressed in Escherichia coli. The recombinant proteins are enzymatically active as homodimers (p66 and p51) as well as a heterodimer p66/p51. The biochemical properties of the FIV RT are very similar to those of the counterpart of the human immunodeficiency virus type 1 in being an RNA-dependent and DNA-dependent DNA polymerase. When a double-stranded DNA containing a small gap of 26 nucleotides was tested, we found a new activity of the FIV RT p66/p51 heterodimer--the cat viral enzyme could perform strand displacement DNA synthesis of approximately 300 bases. The FIV RT homodimer p66 alone could carry out limited strand displacement DNA synthesis, but this activity was stimulated by the p51 subunit at a molar ratio of one molecule of p66 to five molecules of p51. On the other hand, the homodimeric p51 itself was unable to fill a small gap of 26 nucleotides in a double-stranded DNA substrate and was not active by itself in strand displacement DNA synthesis. These data are in agreement with an earlier finding of strand displacement DNA synthesis by human immunodeficiency virus type 1 RT (M. Hottiger, V.N. Podust, ...Continue Reading

References

Jan 1, 1992·Annual Review of Cell Biology·J M Whitcomb, S H Hughes
Jan 1, 1992·Chromosoma·V PodustU Hübscher
Jan 24, 1985·Nature·L RatnerK Baumeister
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R L TalbottJ H Elder
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R A OlmstedP R Johnson
Oct 1, 1982·Journal of Virology·T W Hsu, J M Taylor
Dec 1, 1983·Journal of Virology·J M TaylorJ K Smith
Feb 1, 1994·Antimicrobial Agents and Chemotherapy·T W NorthC K Shih
Sep 2, 1994·Journal of Molecular Biology·P CharneauF Clavel
Jan 1, 1994·Annual Review of Biochemistry·R A Katz, A M Skalka
Mar 11, 1995·Nucleic Acids Research·M HottigerU Hübscher
Jan 1, 1994·Archives of Virology·T MiyazawaT Mikami

❮ Previous
Next ❯

Citations

Jun 1, 2004·The Journal of Biological Chemistry·Christian Lanciault, James J Champoux
Feb 1, 1996·Biological Chemistry Hoppe-Seyler·Y M HenskensA V Nieuw Amerongen
Aug 5, 2006·Medicinal Research Reviews·Donglei YuKuo-Hsiung Lee
May 23, 1998·The Journal of Biological Chemistry·C D Kelleher, J J Champoux
Apr 2, 2010·Cellular and Molecular Life Sciences : CMLS·Alon Herschhorn, Amnon Hizi
Oct 4, 2017·Nucleic Acids Research·Omri MalikAriel Kaplan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.