Feline panleukopenia virus in cerebral neurons of young and adult cats

BMC Veterinary Research
Mutien GariglianyDaniel Desmecht

Abstract

Perinatal infections with feline panleukopenia virus (FPV) have long been known to be associated with cerebellar hypoplasia in kittens due to productive infection of dividing neuroblasts. FPV, like other parvoviruses, requires dividing cells to replicate which explains the usual tropism of the virus for the digestive tract, lymphoid tissues and bone marrow in older animals. In this study, the necropsy and histopathological analyses of a series of 28 cats which died from parvovirus infection in 2013 were performed. Infections were confirmed by real time PCR and immunohistochemistry in several organs. Strikingly, while none of these cats showed cerebellar atrophy or cerebellar positive immunostaining, some of them, including one adult, showed a bright positive immunostaining for viral antigens in cerebral neurons (diencephalon). Furthermore, infected neurons were negative by immunostaining for p27(Kip1), a cell cycle regulatory protein, while neighboring, uninfected, neurons were positive, suggesting a possible re-entry of infected neurons into the mitotic cycle. Next-Generation Sequencing and PCR analyses showed that the virus infecting cat brains was FPV and presented a unique substitution in NS1 protein sequence. Given the rol...Continue Reading

References

Jan 1, 1985·Intervirology·G SieglP Tattersall
Nov 29, 1985·Science·C R ParrishL E Carmichael
Oct 17, 2001·Journal of Virology·A Op De BeeckP Caillet-Fauquet
Aug 9, 2003·Journal of Clinical Microbiology·Angelika UrlPeter Schmidt
May 17, 2005·Research in Veterinary Science·A Url, P Schmidt
Mar 31, 2006·Journal of Virology·Brian D PooleStanley J Naides
Jun 26, 2012·Nucleic Acids Research·Andreas UntergasserSteven G Rozen
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Sep 27, 2014·PloS One·Brejnev Muhizi MuhireDarren Patrick Martin
Jan 16, 2015·Cell Cycle·José M Frade, María C Ovejero-Benito

❮ Previous
Next ❯

Citations

Nov 22, 2017·Veterinary Pathology·Vanessa M PfankucheChristina Puff
Feb 10, 2018·Veterinary Pathology·Chutchai PiewbangAlbert D M E Osterhaus
Jul 3, 2018·Journal of the American Veterinary Medical Association·Federico PorporatoEric Zini
Apr 5, 2019·Virus Genes·Annick LindenMutien Garigliany
Feb 9, 2019·Journal, Genetic Engineering & Biotechnology·Romane A AwadAshraf G Attallah
Aug 21, 2020·Transboundary and Emerging Diseases·René OrtegaCristóbal Castillo-Aliaga
Feb 10, 2021·Transboundary and Emerging Diseases·Federica Di ProfioBarbara Di Martino
Sep 4, 2020·Veterinary Pathology·Surangkanang ChaiyasakSomporn Techangamsuwan
Jul 8, 2020·International Immunopharmacology·Zhihua PeiGuixue Hu
Sep 24, 2021·Veterinary Research Communications·Hasan Abayli, Kezban Can-Sahna

❮ Previous
Next ❯

Datasets Mentioned

BETA
KP769859
KP769860
KM017745

Methods Mentioned

BETA
PCR
PCRs

Software Mentioned

Sequence Demarcation Tool
Geneious

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis