Female-specific insect lethality engineered using alternative splicing

Nature Biotechnology
Guoliang FuLuke Alphey

Abstract

The Sterile Insect Technique is a species-specific and environmentally friendly method of pest control involving mass release of sterilized insects that reduce the wild population through infertile matings. Insects carrying a female-specific autocidal genetic system offer an attractive alternative to conventional sterilization methods while also eliminating females from the release population. We exploited sex-specific alternative splicing in insects to engineer female-specific autocidal genetic systems in the Mediterranean fruit fly, Ceratitis capitata. These rely on the insertion of cassette exons from the C. capitata transformer gene into a heterologous tetracycline-repressible transactivator such that the transactivator transcript is disrupted in male splice variants but not in the female-specific one. As the key components of these systems function across a broad phylogenetic range, this strategy addresses the paucity of sex-specific expression systems (e.g., early-acting, female-specific promoters) in insects other than Drosophila melanogaster. The approach may have wide applicability for regulating gene expression in other organisms, particularly for combinatorial control with appropriate promoters.

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Gossen, H Bujard
Jan 1, 1992·Medical and Veterinary Entomology·D A LindquistM J Hall
Dec 11, 1999·Brain Research. Brain Research Protocols·O StossS Stamm
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·J C Heinrich, M J Scott
Dec 29, 2000·The Journal of Biological Chemistry·O StossS Stamm
Jan 6, 2001·Journal of Economic Entomology·Paul Schliekelman, F Gould
Apr 23, 2002·Nature Reviews. Genetics·Luca CartegniAdrian R Krainer
Sep 13, 2002·Insect Biochemistry and Molecular Biology·Luke Alphey
Oct 31, 2002·Development·Antonio Baonza, Matthew Freeman
Dec 17, 2002·Nature Biotechnology·Carsten Horn, Ernst A Wimmer
Dec 18, 2002·Genetica·Giuseppe SacconeL C Polito
Mar 11, 2003·Annual Review of Biochemistry·Douglas L Black
Dec 4, 2003·Annual Review of Entomology·Fred Gould, Paul Schliekelman
Oct 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jung W ParkBrenton R Graveley
Apr 9, 2005·Nature Biotechnology·Ernst A Wimmer
May 14, 2005·Journal of Economic Entomology·Frantisek MarecGerald Franz
Jul 7, 2005·Genetics·Attilio PaneCatello Polito
Oct 1, 1997·Development Genes and Evolution·C Tortiglione, M Bownes

Citations

Nov 19, 2013·Insect Biochemistry and Molecular Biology·Xiu-Ye WangYong-Zhen Xu
Apr 7, 2010·Nature Methods·Andrea CalixtoMartin Chalfie
Oct 16, 2009·Proceedings of the National Academy of Sciences of the United States of America·Marc F ScheteligErnst A Wimmer
Feb 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Guoliang FuLuke Alphey
Jun 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Marc F Schetelig, Alfred M Handler
Apr 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Anjiang TanYongping Huang
Sep 4, 2009·Vector Borne and Zoonotic Diseases·Luke AlpheyStephen L Dobson
Apr 18, 2009·Vector Borne and Zoonotic Diseases·Luke Alphey
Sep 13, 2011·Nucleic Acids Research·Julian P VenablesFrançois Juge
Oct 5, 2012·Clinical Microbiology Reviews·Tonya M ColpittsErol Fikrig
Dec 16, 2009·Malaria Journal·Philippos A PapathanosAndrea Crisanti
Dec 16, 2009·Malaria Journal·Flaminia CatterucciaErnst A Wimmer
Feb 10, 2012·PLoS Neglected Tropical Diseases·R Guy ReevesFloyd A Reed
Jul 18, 2012·PLoS Neglected Tropical Diseases·Geneviève LabbéLuke Alphey
Sep 21, 2011·PloS One·Gregory S SimmonsLuke Alphey
Jul 20, 2013·Ecological Applications : a Publication of the Ecological Society of America·Ronald E ThresherNicholas J Bax
Aug 15, 2014·Proceedings. Biological Sciences·Philip T LeftwichLuke Alphey
Dec 14, 2011·Annual Review of Entomology·Malcolm J Fraser
Sep 30, 2010·Journal of Genetics·Tarig Dafa'allaLuke Alphey
Sep 23, 2009·Journal of Medical Entomology·Felix D GuerreroBruce A Roe
Apr 9, 2009·Journal of Medical Entomology·Felix D GuerreroLane D Foil
Apr 19, 2016·Evolutionary Applications·Philip T LeftwichTracey Chapman
Nov 10, 2012·Insect Biochemistry and Molecular Biology·Christian E OgaugwuErnst A Wimmer
Aug 4, 2012·Insect Biochemistry and Molecular Biology·Marc F Schetelig, Alfred M Handler
Aug 23, 2012·Gene·Olga KelemenS Stamm
Sep 19, 2009·Current Opinion in Biotechnology·Marcel Tigges, Martin Fussenegger
Jul 8, 2009·Drug Discovery Today·Wilfried Weber, Martin Fussenegger
Jan 10, 2008·Trends in Parasitology·Alasdair J NisbetRobin B Gasser
Oct 6, 2007·Insect Biochemistry and Molecular Biology·Kirsty C CondonLuke Alphey
Sep 27, 2007·Insect Molecular Biology·Kirsty C CondonLuke Alphey
Mar 27, 2010·Insect Molecular Biology·Daisuke S YamamotoS Yoshida
Nov 21, 2013·Acta Tropica·Kostas BourtzisJeremie R L Gilles
Jun 15, 2014·Insect Biochemistry and Molecular Biology·Fang LiMaxwell J Scott
Nov 16, 2010·Journal of Theoretical Biology·Nina AlpheyLuke Alphey
Sep 17, 2013·Insect Biochemistry and Molecular Biology·Sanyuan MaQingyou Xia
Mar 24, 2015·Biomedical Reports·Yan WangXiao-Zhong Wang
Nov 11, 2008·Cell Host & Microbe·Olle TereniusAnthony A James
Mar 26, 2009·Chemistry & Biology·Wilfried Weber, Martin Fussenegger
Sep 23, 2014·International Journal for Parasitology·R M SandemanV M Bowles
May 3, 2016·Insect Molecular Biology·Marc F ScheteligAlfred M Handler
Nov 7, 2016·Journal of Theoretical Biology·Benjamin Watkinson-Powell, Nina Alphey
Nov 6, 2015·Scientific Reports·Ying Yan, Maxwell J Scott
Sep 18, 2012·Revista Do Instituto De Medicina Tropical De São Paulo·André Barretto Bruno Wilke, Mauro Toledo Marrelli
Nov 30, 2011·Nature Reviews. Genetics·Wilfried Weber, Martin Fussenegger
Dec 18, 2019·Archives of Insect Biochemistry and Physiology·Konstantina T TsoumaniPhilippos A Papathanos
Aug 29, 2019·Pest Management Science·Yaohui WangChangying Niu
Feb 25, 2020·Archives of Insect Biochemistry and Physiology·Maria-Eleni Gregoriou, Kostas D Mathiopoulos
Feb 6, 2020·G3 : Genes - Genomes - Genetics·Katherine E KnudsenMaxwell J Scott
Jun 26, 2018·Agricultural and Forest Entomology·Nina Alphey, Michael B Bonsall
Dec 1, 2015·Systems and Synthetic Biology·Johannes StarkbaumPeter Dabrock
Jun 18, 2017·Biological Invasions·Tim Harvey-SamuelLuke Alphey
May 2, 2020·Nature Communications·Nikolay P KandulOmar S Akbari
Jun 27, 2020·Biological Reviews of the Cambridge Philosophical Society·Petr PyšekDavid M Richardson
Jul 3, 2013·Pathogens and Global Health·Luke AlpheyCamilla Beech

Related Concepts

Transformer protein, Drosophila
Subfertility, Female
Insect Control
Nucleolar Proteins
Neomalthusianism
Topicycline
Transcription, Genetic
Nested Transcripts
Ceratitis capitata

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