Female X-chromosome mosaicism for gp91phox expression diversifies leukocyte responses during endotoxemia.

Critical Care Medicine
Rachna ChandraZoltán Spolarics

Abstract

To test the hypothesis, using an animal model, whether female X-chromosome mosaicism for inflammatory gene expression could contribute to the gender dimorphic response during the host response. X-chromosome-linked genetic polymorphisms present a unique biological condition because females display heterozygous cellular mosaicism, due to the fact that either the maternal or the paternal X chromosomes are inactivated in each individual cell in females. This is in contrast with the conditions in males who carry exclusively the maternal X chromosome. Prospective, randomized, laboratory investigation. University research laboratory. Female mice deficient, heterozygous (mosaic) or WT for the X-linked gp91phox. We compared selected inflammatory markers among heterozygous (mosaics), WT and homozygous deficient animals in response to in vivo lipopolysaccharide (Escherichia coli, 20 mg/kg body weight). To test individual mosaic subpopulations of polymorphonuclear neutrophil responses, we also developed a flow cytometry assay that identifies the active parental X chromosomes in individual cells, using gp91phox expression as a marker. Heterozygous mosaic mice presented white blood cell trafficking patterns similar to that observed in WT mic...Continue Reading

References

Feb 1, 1996·Nature Medicine·J L AbkowitzP Guttorp
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·T Goto, M Monk
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiao-pei GaoAsrar B Malik
Aug 6, 2002·Molecular and Cellular Endocrinology·Elizabeth J KovacsMeredith S Gregory
Jun 6, 2003·Endocrine Reviews·Peter Y LiuDavid J Handelsman
Jan 7, 2004·Nature Reviews. Genetics·Stephen F Schaffner
Apr 17, 2004·Shock·Christopher J GannonLena M Napolitano
Jan 7, 2005·Seminars in Reproductive Medicine·Chi Chiung Grace Chen, C Richard Parker
Jan 22, 2005·Trends in Genetics : TIG·Keith E Latham
Dec 24, 2005·Shock·Daniel G Remick, Peter A Ward
Mar 23, 2006·JAMA : the Journal of the American Medical Association·Barbara R Migeon
Apr 18, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Edwin A DeitchCarl J Hauser
Jun 22, 2006·Endocrine, Metabolic & Immune Disorders Drug Targets·Mashkoor A ChoudhryIrshad H Chaudry
Jul 19, 2006·Genes & Development·Edith Heard, Christine M Disteche
Aug 1, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Shaolong YangIrshad H Chaudry
Jan 6, 2007·Biochemical and Biophysical Research Communications·Claudia PiccoliNazzareno Capitanio
Sep 1, 2007·Antioxidants & Redox Signaling·Ren-Feng Guo, Peter A Ward
Mar 21, 2008·Journal of Leukocyte Biology·Rachna ChandraZoltán Spolarics
Oct 1, 2008·The Journal of Biological Chemistry·S Courtney FraschDonna L Bratton
Nov 27, 2009·Genome Medicine·David G Clayton

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Citations

Dec 23, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Louise Tzung-Harn HsiehLiliana Schaefer
Nov 7, 2013·Virulence·Martin K AngeleIrshad H Chaudry
Sep 5, 2019·Expert Review of Proteomics·Kristine Y DeLeon-Pennell, Merry L Lindsey
Mar 14, 2020·Minerva medica·Elena BarbagelataPaolo Solidoro
Apr 20, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rachna ChandraZoltán Spolarics
Jul 19, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Manuel Ponce-AlonsoRosa Del Campo

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