Femtomolar electrochemical detection of DNA hybridization using hollow polyelectrolyte shells bearing silver nanoparticles

Analytical Chemistry
Patsamon RijiravanichWerasak Surareungchai

Abstract

The preparation, and use as electrochemical labels, of polyelectrolyte shells bearing Ag nanoparticles is described. Their potential for highly sensitive detection is demonstrated. The shells are prepared by layer-by-layer self-assembly around templates (500 nm diameter) which are then dissolved. The shells can be opened and closed by adjustment of solution pH, and this process is utilized to encapsulate Ag nanoparticles, chiefly by adsorption to the inner walls of the capsules. Based on absorbance, TEM and voltammetric measurements, the highest loading achieved is approximately 78 Ag particles per capsule. The Ag capsules are used via biotin-avidin binding as labels for the detection of DNA hybridization, following acid dissolution and then detection of the Ag (+) by ASV. A 30-mer sequence specific to Escherichia coli is measured at DNA-modified screen-printed electrodes with a detection limit of approximately 25 fM, which corresponds to the detection of 4.6 fg ( approximately 3 x 10 (5) molecules) in the 20 microL analyte sample. A 200 fM target containing a single mismatch gives a significantly (<74%) lower response than 200 fM of complementary target; 60 pM of noncomplementary target gives a negligible response.

References

Apr 10, 1987·Nucleic Acids Research·S F WolfK Jacobs
Sep 1, 1993·Analytical Chemistry·K M Millan, S R Mikkelsen
Jun 24, 1999·Bioelectrochemistry and Bioenergetics·J WangE Palecek
Apr 15, 2000·Science·J FritzJ K Gimzewski
Mar 13, 2003·Journal of the American Chemical Society·Joseph WangArben Merkoçi
Oct 2, 2003·Nature Biotechnology·T Gregory DrummondJacqueline K Barton
Nov 18, 2003·Analytical Chemistry·Joseph WangQiyu Zhu
Aug 11, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Andre G SkirtachGleb B Sukhorukov
Aug 11, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Christophe Déjugnat, Gleb B Sukhorukov
Nov 13, 2004·Angewandte Chemie·Eugenii Katz, Itamar Willner
Oct 14, 2005·The Analyst·Ralf Lucklum
Jul 21, 2006·The Journal of Physical Chemistry. B·Alexandra S AngelatosFrank Caruso
Jul 21, 2006·The Journal of Physical Chemistry. B·Ramón A Alvarez-PueblaRicardo F Aroca
Sep 4, 1998·Angewandte Chemie·Edwin DonathHelmuth Möhwald

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Citations

May 27, 2010·The Analyst·Eli G Hvastkovs, Daniel A Buttry
Nov 29, 2011·Journal of Proteomics·Miguel Larguinho, Pedro V Baptista

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